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Chromatographic Fingerprint Analysis of Exocarpium Citri Grandis by High-Performance Liquid Chromatography Coupled with Diode-Array Detector

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Abstract

A newly validated HPLC fingerprint method has been developed for the identification and quality assessment of Exocarpium Citri Grandis. Twenty-three batches of Exocarpium Citri Grandis collected or purchased from different localities in China were investigated. The analyses were conducted on a Diamonsil C18 column (250 mm × 4.6 mm, 5-μm particle) preceded by a Diamonsil C18 guard column (10 mm × 4.6 mm, 5-μm particle). Mobile phases were composed of 0.5 % acetic acid (A) and methanol (B), with a flow rate of 1 mL/min under a gradient elution. The wavelength was set at 254 nm. The common fingerprint profile was established with professional analytical software. Common peaks were further identified using LC-DAD-MS/MS. Chemometric methods including similarity calculation and hierarchical clustering analysis were performed to differentiate the 23 batches of Exocarpium Citri Grandis samples. Fifteen batches of samples had a high similarity (more than 0.9) and the overall 23 batches of samples were divided into two clusters. This method presented good precision, repeatability, and stability; therefore, it would be a reliable and useful approach for the quality control of Exocarpium Citri Grandis.

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Acknowledgments

This work was supported by the Guangdong Province Special Major Science and Technology Project (no. 2011A080503001) and the Industry/University Research Cooperation Program from Science and Technology Department of Qingyuang City (no. 2012D021211001).

Conflict of Interest

Xiaoxue Yu has no conflict of interest. Qundi Liu has no conflict of interest. Zhisheng Xie has no conflict of interest. Shingchung Lam has no conflict of interest. Xinjun Xu has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Sun Yat-sen University, 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou, 510006, China

    Xiaoxue Yu, Qundi Liu, Zhisheng Xie, Shingchung Lam & Xinjun Xu

  2. Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou, 510006, China

    Xinjun Xu

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  1. Xiaoxue Yu
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  2. Qundi Liu
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  3. Zhisheng Xie
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  4. Shingchung Lam
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  5. Xinjun Xu
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Correspondence to Xinjun Xu.

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Yu, X., Liu, Q., Xie, Z. et al. Chromatographic Fingerprint Analysis of Exocarpium Citri Grandis by High-Performance Liquid Chromatography Coupled with Diode-Array Detector. Food Anal. Methods 8, 1868–1875 (2015). https://doi.org/10.1007/s12161-014-0071-9

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  • DOI: https://doi.org/10.1007/s12161-014-0071-9

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