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Speciation Determination of Selenium in Seafood by High-Performance Ion-Exchange Chromatography-Hydride Generation-Atomic Fluorescence Spectrometry

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Abstract

A high-performance ion-exchange chromatography coupled with hydride generation-atomic fluorescence spectrometry (HPIEC-HG-AFS) method was developed for simultaneous speciation of selenium in seafood. Three selenium species including of selenocystine (Se-Cys), selenome-thionine (Se-Met), and selenite Se(IV) were separated on an anion-exchange column (PRP-X100) with eluent of 30 mM NH4H2PO4 and methanol (39:1, v/v) in 10 min at the flow rate of 1.0 mL min−1. Variables affecting the HG-AFS detection of selenium species were optimized. The optimum conditions found were the following: reducing agent, 2.0 % of KBH4, and 5.0 % of HCl; lamp current, 90 mA; photomultiplier tube voltage, 280 V; flow rate of carrier gas, 300 mL min−1; and shielding gas, 800 mL min−1. Under the optimized conditions, the good linearity of calibration curves (R 2 > 0.999) between signal of fluorescence and concentration of selenium species was obtained in the range of detection limits (DLs), 80 μg L−1, and the DLs of Se-Cys, Se-Met, Se(IV) were 1.66, 0.990, 1.10 μg L−1, respectively. The repeatability of the method, expressed as relative standard deviation, was less than 5.0 % (n = 10), and the average recoveries for spiked test were from 87.3 to 103 % for three analytes in real seafood samples. The developed HPIEC-HG-AFS method was successfully applied for the speciation of selenium in seafood samples.

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Acknowledgments

The research project was jointly supported by the National Natural Science Foundation of China (21207102; 21477088) and the Qianjiang Talents Plan of Science & Technology Department of Zhejiang Province (2013R10067).

Conflict of Interest

Xiaoxue Xie declares that he has no conflict of interest.

Chuchu Feng declares that he has no conflict of interest.

Mingde Ye declares that he has no conflict of interest.

Chengjun Wang declares that he has no conflict of interest.

All institutional and national guidelines for the care and use of laboratory animals were followed.

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Authors and Affiliations

  1. College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, 325035, China

    Xiaoxue Xie, Chuchu Feng, Mingde Ye & Chengjun Wang

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  1. Xiaoxue Xie
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  2. Chuchu Feng
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  3. Mingde Ye
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  4. Chengjun Wang
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Correspondence to Chengjun Wang.

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Xie, X., Feng, C., Ye, M. et al. Speciation Determination of Selenium in Seafood by High-Performance Ion-Exchange Chromatography-Hydride Generation-Atomic Fluorescence Spectrometry. Food Anal. Methods 8, 1739–1745 (2015). https://doi.org/10.1007/s12161-014-0055-9

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  • DOI: https://doi.org/10.1007/s12161-014-0055-9

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