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Enantioseparation and Enantioselective Phytotoxicity of Glufosinate Ammonium on Catechin Biosynthesis in Wheat

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Abstract

The enantioselective phytotoxicity of chiral pesticides is a new area of research in phyto-biochemical studies. Glufosinate (GL) is a chiral herbicide, comprised of the two enantiomers. The aim of this study was to investigate the enantioselective phytotoxicity of glufosinate ammonium enantiomers on root and shoot dry weight as well as catechin biosynthesis of wheat seedlings. Separation of GL enantiomers was achieved by using high-performance liquid chromatography on Eurocel 01 column. The optical rotation of separated enantiomers confirmed the purity of each isomer. The I50 parameter, which is the concentration resulting in a 50 % inhibition in root and shoot dry weight estimated using dose–response curves, showed that (+)-GL was 4.2 times more active than the (−)-GL enantiomer. It demonstrated that the effect of enantioselectivity of GL on catechin biosynthesis in wheat shoots was sensible, while the effect of enantioselectivity of GL in catechin content in wheat roots was not distinct.

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Conflict of Interest

Mohammad Khodadady declares that she has no conflict of interest. Mohammad Kazem Ramezani declares that he has no conflict of interest. Vehideh Mahdavi declares that she has no conflict of interest. Alireza Ghassempour declares that she has no conflict of interest. Hassan Y. Aboul-Enein declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, P.O. Box: 19835-389, G.C. Evin, Tehran, Iran

    Mohammad Khodadady, Vahideh Mahdavi & Alireza Ghassempour

  2. Pesticides Research Department, Iranian Research Institute of Plant Protection, P.O. Box 1454-19395, Tehran, Iran

    Mohammad Kazem Ramezani

  3. Pharmaceutical and Drug Industries Research Division, Pharmaceutical and Medicinal Chemistry Department, National Research Centre, Dokki, Cairo, 12311, Egypt

    Hassan Y. Aboul-Enein

Authors
  1. Mohammad Khodadady
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  2. Mohammad Kazem Ramezani
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  3. Vahideh Mahdavi
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  4. Alireza Ghassempour
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  5. Hassan Y. Aboul-Enein
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Correspondence to Alireza Ghassempour or Hassan Y. Aboul-Enein.

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Khodadady, M., Ramezani, M.K., Mahdavi, V. et al. Enantioseparation and Enantioselective Phytotoxicity of Glufosinate Ammonium on Catechin Biosynthesis in Wheat. Food Anal. Methods 7, 747–753 (2014). https://doi.org/10.1007/s12161-013-9677-6

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  • DOI: https://doi.org/10.1007/s12161-013-9677-6

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