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Preconcentration and Simultaneous Analysis of Benzimidazole Anthelmintics in Milk Samples by Ultrasound-Assisted Surfactant-Enhanced Emulsification Microextraction and High-Performance Liquid Chromatography

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Abstract

The proposed ultrasound-assisted surfactant-enhanced emulsification microextraction (UASEME) coupled with high-performance liquid chromatography-photodiode array detection (HPLC-PDA) has been developed for the preconcentration and simultaneous analysis of five benzimidazole anthelmintics. Dichloromethane (extraction solvent) and Triton X-114 (emulsifier) was used for extraction of the target analytes. The parameters affecting the extraction efficiency were investigated and optimized. Under the optimum conditions, linearity was in the range from 10 to 150 μg L−1 with good coefficients of determination (R 2) higher than 0.994. Preconcentration factors were obtained up to 60, corresponding to limits of detection range of 1.8 − 3.6 μg L−1. Intra-day (n = 5) and inter-day (n = 4 × 3) precisions were obtained with relative standard deviation of retention time and peak area below 0.8 and 9.2 %, respectively. Good recoveries for the spiked target anthelmintics at different concentrations (e.g., 20, 50, and 100 μg L−1) of milk samples were obtained in 72.5–113.5 %. The results demonstrated that the proposed UASEME-HPLC-PDA can be used as an alternative powerful method for the simultaneous determination of the target analytes in milk samples.

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Acknowledgments

This research was financially supported by the Thailand Research Fund (TRF), the Commission on Higher Education (CHE), and Rajamangala University of Technology Isan, Khon Kaen Campus through the TRF-CHE Research Grant for New Scholars under grant no. MRG5480135. The authors would like to acknowledge Department of Chemistry, Faculty of Science, Khon Kaen University for providing deionized water. The authors also thank Miss Jittraporn Phaphakdee and Miss Sudathip Supaket for their assistance in the laboratory.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Engineering, Rajamangala University of Technology Isan, Khon Kaen Campus, Khon Kaen, 40000, Thailand

    Yanawath Santaladchaiyakit

  2. Materials Chemistry Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Supalax Srijaranai

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  1. Yanawath Santaladchaiyakit
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  2. Supalax Srijaranai
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Correspondence to Yanawath Santaladchaiyakit.

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Santaladchaiyakit, Y., Srijaranai, S. Preconcentration and Simultaneous Analysis of Benzimidazole Anthelmintics in Milk Samples by Ultrasound-Assisted Surfactant-Enhanced Emulsification Microextraction and High-Performance Liquid Chromatography. Food Anal. Methods 6, 1551–1560 (2013). https://doi.org/10.1007/s12161-013-9569-9

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