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A Rapid Colorimetric Detection of Melamine in Raw Milk by Unmodified Gold Nanoparticles

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Abstract

We report a rapid, visual, facile colorimetric detection of melamine in raw milk by unmodified gold nanoparticles (AuNPs). AuNPs can be induced by melamine from dispersion to aggregation rapidly, along with the color change from red to blue (or purple). After the optimization of reaction conditions, the whole analysis only needs about 20 min including the simple pretreatment we designed. The analysis can be observed by the naked eye and simple to operate without sophisticated instruments. In the raw milk, the present limit of detection for melamine is down to ∼70 ppb. Furthermore, we explore the interaction principle between melamine and citrate-stabilized AuNPs in this work and establish the foundation for modifying the AuNPs and optimizing the system of AuNPs–melamine in the future.

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Acknowledgments

This work was supported by the National High-Tech Research and Development Plan (2012AA101405), the Special Fund for Agro-Scientific Research in the Public Interest of China (200903056), the National Natural Science Foundation of China (31071860), and the National key Technology R&D Program (2010BAK69B18).

Conflict of Interest

There are no conflicts of interest.

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Authors and Affiliations

  1. School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, China

    Hai-bo Xing, Yuan-gen Wu, Shen-shan Zhan & Pei Zhou

  2. Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, China

    Hai-bo Xing, Yuan-gen Wu, Shen-shan Zhan & Pei Zhou

  3. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Pei Zhou

  4. Bor S. Lug Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, China

    Pei Zhou

  5. The Center for Food Nutrition and Safety, Shanghai Jiao Tong University, Shanghai, China

    Hai-bo Xing & Pei Zhou

Authors
  1. Hai-bo Xing
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  2. Yuan-gen Wu
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  3. Shen-shan Zhan
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  4. Pei Zhou
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Correspondence to Pei Zhou.

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Xing, Hb., Wu, Yg., Zhan, Ss. et al. A Rapid Colorimetric Detection of Melamine in Raw Milk by Unmodified Gold Nanoparticles. Food Anal. Methods 6, 1441–1447 (2013). https://doi.org/10.1007/s12161-013-9562-3

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  • DOI: https://doi.org/10.1007/s12161-013-9562-3

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