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Automating a 96-Well Microtiter Plate Assay for Quick Analysis of Chemically Available Lysine in Foods

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Abstract

A new method for quick analysis of available lysine content in different food products has been developed by automating a 96-well microtiter plate assay. Although manual fluorometric methods validated in order to determinate available lysine content already existed for this compound, the benefits of applying appropriate automation should provide continuous operation, increased precision, an affordable electronic audit trail, and significantly reduced time and reagent consumption. The objective of this work was to adapt the ortho-phthaldialdehyde (OPA) fluorometric method to an automated workstation. Considerable effort went into developing and validating an automated method. The analytical parameters of linearity (r = 0.999), the precision of the method (relative standard deviations = 2.8–3.0 % for the different samples), and the results of the comparison with the corresponding OPA manual fluorometric method show that the studied method is useful for the measurement of available lysine in several food products from different natural origins such as liquid foods (soy, oat, quinoa beverages, and ultra-high temperature/sterilized milk) and powdered samples (powdered adapted, powdered follow-up, and junior milk infant formulas) with reduced time and reagent consumption.

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Acknowledgments

This research project was supported by the Spanish Ministry of Science and Technology and European Regional Development Funds (AGL2010-22206-C02-01). Barba, F.J received a contract employment from this project to carry out the study. Carbonell-Capella, J.M. holds an award from the Spanish Ministry of Education (AP2010-2546).

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Authors and Affiliations

  1. Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Cientificas (CSIC), Avda. Agustín Escardino, 7., 46980, Paterna, Spain

    Francisco J. Barba

  2. Department of Nutrition and Food Chemistry, Universitat de València, Avda. Vicent Andrés Estellés, s/n., 46100, Burjassot, Spain

    Juana M. Carbonell-Capella, María J. Esteve & Ana Frígola

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  1. Francisco J. Barba
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  2. Juana M. Carbonell-Capella
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  3. María J. Esteve
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  4. Ana Frígola
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Correspondence to Ana Frígola.

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Barba, F.J., Carbonell-Capella, J.M., Esteve, M.J. et al. Automating a 96-Well Microtiter Plate Assay for Quick Analysis of Chemically Available Lysine in Foods. Food Anal. Methods 6, 1258–1264 (2013). https://doi.org/10.1007/s12161-012-9535-y

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  • DOI: https://doi.org/10.1007/s12161-012-9535-y

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