Abstract
Mycotoxins are secondary metabolites produced by fungi, such as Fusarium, Penicillium, and Aspergillus, which are toxic to humans with high risk factors and pose a significant threat to human health. This study was focused mostly on well-known mycotoxins, such as aflatoxins (AFB1, AFB2, AFG1 and AFG2), fumonisin (FB1, FB2), deoxynivalenol (DON), zearalenone (ZON), ochratoxin A, T-2 and HT-2, in grains. The multi-mycotoxin methods developed in this study utilise an analysis of mycotoxin through liquid chromatography tandem mass spectrometry (LC-MS/MS), which can significantly improve sample analysis efficiency. The Myco6in1™ immunoaffinity column was used for purification to reduce interference from the substrate. Gradient separation to obtain the best peak shift was conducted using solvent with 0.1 % formic acid in deionised water and methanol, and gradient separation was performed on an ACQUITY BEH C18 column chromatograph. The recovery rate test for each toxin using substrates such as rice, peanut, wheat and maize mostly indicated good average recovery rates between 70 % and 120 % and the coefficient of variation mostly under 15 %. The limits of quantification (LOQ) identified by this method are less than 5 ng/g in most toxins, except for 20 ng/g in FB1and FB2. This method can rapidly and simultaneously analyse 11 mycotoxins in 9 min. It can be applied for the practical examination of mycotoxins in food to protect public health.
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Tang, Y.Y., Lin, H.Y., Chen, Y.C. et al. Development of a Quantitative Multi-Mycotoxin Method in Rice, Maize, Wheat and Peanut Using UPLC-MS/MS. Food Anal. Methods 6, 727–736 (2013). https://doi.org/10.1007/s12161-012-9473-8
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DOI: https://doi.org/10.1007/s12161-012-9473-8