Abstract
Sea urchin roe (Paracentrotus lividus) is an excellent biomarker, and it has been used as a test for toxicology study of chemical pollution of restricted marine areas; in fact, unlike mussels which filter water, it feeds mainly on micro- and macroalgae. Therefore, it is not influenced by the sea tide and meets better the situation of local pollution. A modified Quick Easy Cheap Effective Rugged Safe extraction method coupled with gas chromatography ion trap mass spectrometry analysis has been used to set up a method for the determination of 17 polycyclic aromatic hydrocarbons (PAHs) in P. lividus sea urchin roe. A method validation was performed, and the following parameters have been determined: instrument limit of detection (LOD) and of quantification (LOQ), precision, recovery, and linearity. The method carried out showed good LOD under 1.5 μg/kg and LOQ under 5 μg/kg for all PAHs studied, and these values were in close agreement with quality criteria described in the Commission Regulation (EC) no. 333/2007 concerning the PAH benzo[a]pyrene in foodstuffs. Precision and linearity were in accordance with EC SANCO/10684/2009 values. For all PAHs, recoveries ranged from 72% to 119%, with RSD ranging from 3% to 17% in the most unfavorable case.
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Angioni, A., Porcu, L., Secci, M. et al. QuEChERS Method for the Determination of PAH Compounds in Sardinia Sea Urchin (Paracentrotus lividus) Roe, Using Gas Chromatography ITMS-MS Analysis. Food Anal. Methods 5, 1131–1136 (2012). https://doi.org/10.1007/s12161-011-9353-7
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DOI: https://doi.org/10.1007/s12161-011-9353-7