Glucose Measurement in the Presence of Tea Polyphenols

Abstract

The accuracy of several commonly used methods for glucose measurement in the presence of tea polyphenols (TPLs) was investigated since TPLs (as a representative of bioactive polyphenols) and glucose often co-exist in experiments exploring TPLs’ effect on carbohydrate metabolism. The results from a model system containing only glucose and TPLs showed a TPLs’ amount-dependent variation of measured glucose concentration with a relative error (RE) of 5.0% to 35.5% when a dinitrosalicylic acid (DNS) method was used, and for glucose oxidase/peroxidase assay, the results showed a decreased content of glucose with a RE from 56.7% to 102.7%. When a hexose/kinase (HK) method was employed to quantify the glucose content, the accuracy (RE from 0.57% to 4.7%) was comparable to the result obtained by a high performance liquid chromatography (HPLC) method (RE = 0.7–3.0%) that was used as the standard control. Starch digestion experiment further demonstrated the invalidity of DNS method and the accuracy of HK method. Thus, the HK method with its accuracy and convenience is the preferred method for glucose measurement in the presence of TPLs or other bioactive polyphenols.

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References

  1. Babu PV, Liu D (2008) Green tea catechins and cardiovascular health: an update. Curr Med Chem 15(18):1840–1850

    Article  CAS  Google Scholar 

  2. Chen Z-Y, Zhu QY, Tsang D, Huang Y (2000) Degradation of green tea catechins in tea drinks. J Agric Food Chem 49(1):477–482

    Article  Google Scholar 

  3. Englyst HN, Kingman SM, Cummings JH (1992) Classification and measurement of nutritionally important starch fractions. Eur J Clin Nutr 46(Suppl 2):S33–S50

    Google Scholar 

  4. Finch PR, Yuen R, Schachter H, Moscarello MA (1969) Enzymic methods for the micro assay of d-mannose, d-glucose, d-galactose, and l-fucose from acid hydrolyzates of glycoproteins. Anal Biochem 31:296–305

    Article  CAS  Google Scholar 

  5. Hanhineva K, Torronen R, Bondia-Pons I, Pekkinen J, Kolehmainen M, Mykkanen H, Poutanen K (2010) Impact of dietary polyphenols on carbohydrate metabolism. Int J Mol Sci 11(4):1365–1402

    Article  CAS  Google Scholar 

  6. Hara Y, Honda M (1990) The inhibition of alpha-amylase by tea polyphenols. Agri Bio Chem 54(8):1939–1945

    Article  CAS  Google Scholar 

  7. Higdon JV, Frei B (2003) Tea catechins and polyphenols: health effects, metabolism, and antioxidant functions. Crit Rev Food Sci Nutr 43(1):89–143

    Article  CAS  Google Scholar 

  8. Kobayashi Y, Suzuki M, Satsu H, Arai S, Hara Y, Suzuki K, Miyamoto Y, Shimizu M (2000) Green tea polyphenols inhibit the sodium-dependent glucose transporter of intestinal epithelial cells by a competitive mechanism. J Agric Food Chem 48(11):5618–5623

    Article  CAS  Google Scholar 

  9. Koh LW, Wong LL, Loo YY, Kasapis S, Huang D (2010) Evaluation of different teas against starch digestibility by mammalian glycosidases. J Agric Food Chem 58(1):148–154

    Article  CAS  Google Scholar 

  10. Kwon Y-I, Apostolidis E, Shetty K (2008) Inhibitory potential of wine and tea against α-amylase and α-glucosidase for management of hyperglycemia linked to type 2 diabetes. J Food Biochem 32(1):15–31

    Article  CAS  Google Scholar 

  11. Liu J, Wang M, Peng S, Zhang G (2011) Effect of green tea catechins on the postprandial glycemic response to starches differing in amylose content. J Agric Food Chem 59(9):4582–4588

    Article  CAS  Google Scholar 

  12. Matsui T, Tanaka T, Tamura S, Toshima A, Tamaya K, Miyata Y, Tanaka K, Matsumoto K (2007) alpha-Glucosidase inhibitory profile of catechins and theaflavins. J Agric Food Chem 55(1):99–105

    Article  CAS  Google Scholar 

  13. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31(3):426–428

    Article  CAS  Google Scholar 

  14. Neilson AP, Hopf AS, Cooper BR, Pereira MA, Bomser JA, Ferruzzi MG (2007) Catechin degradation with concurrent formation of homo- and heterocatechin dimers during in vitro digestion. J Agric Food Chem 55(22):8941–8949

    Article  CAS  Google Scholar 

  15. Ruch RJ, Cheng SJ, Klaunig JE (1989) Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 10(6):1003–1008

    Article  CAS  Google Scholar 

  16. Sang S, Lee M-J, Hou Z, Ho C-T, Yang CS (2005) Stability of tea polyphenol (−)-epigallocatechin-3-gallate and formation of dimers and epimers under common experimental conditions. J Agric Food Chem 53(24):9478–9484

    Article  CAS  Google Scholar 

  17. Trinder P (1969) Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. J Clin Pathol 22(2):158–161

    Article  CAS  Google Scholar 

  18. Wang H, Provan GJ, Helliwell K (2003) HPLC determination of catechins in tea leaves and tea extracts using relative response factors. Food Chem 81(2):307–312

    Article  CAS  Google Scholar 

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Funding Sources

The current investigation was supported by National Natural Science Foundation of China (project no. 21076095).

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Correspondence to Genyi Zhang.

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Xu, H., Leng, X., Wang, M. et al. Glucose Measurement in the Presence of Tea Polyphenols. Food Anal. Methods 5, 1027–1032 (2012). https://doi.org/10.1007/s12161-011-9335-9

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Keywords

  • Glucose
  • Tea polyphenols
  • DNS method
  • Glucose oxidase method
  • Hexokinase method
  • HPLC method