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A Simple Solid-Phase Extraction Coupled to High-Performance Liquid Chromatography–UV Detection for Quantification of Pyrethroid Residues in Fruits and Vegetables

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Abstract

A method for determination of pyrethroid insecticide residues in fruits and vegetables based on a simple extraction using acetonitrile followed by solid-phase extraction and high-performance liquid chromatography-UV is described. Separation of pyrethroids was carried out on an Atlantis C18 column using an isocratic mobile phase of 75% acetonitrile with a flow rate of 1.0 mL min−1 and UV detection at 225 nm. Matrix effect was overcome using matrix-matched standards for building up the calibration graphs. The linear ranges were established between 0.01 and 0.50 μg mL−1. The concentration of pyrethroids was enhanced with the factors up to 1,025 folds. The method has been successfully applied in a monitoring of pyrethroid residues contaminated in fruits and vegetables.

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Acknowledgment

Financial support from the Center for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education, Ministry of Education, is gratefully acknowledged.

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  1. Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Niyom Wongsa & Rodjana Burakham

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  1. Niyom Wongsa
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  2. Rodjana Burakham
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Correspondence to Rodjana Burakham.

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Wongsa, N., Burakham, R. A Simple Solid-Phase Extraction Coupled to High-Performance Liquid Chromatography–UV Detection for Quantification of Pyrethroid Residues in Fruits and Vegetables. Food Anal. Methods 5, 849–855 (2012). https://doi.org/10.1007/s12161-011-9317-y

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  • DOI: https://doi.org/10.1007/s12161-011-9317-y

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