Abstract
In this paper, a cloud-point extraction method was developed for the determination of five anthraquinone derivatives in Chang-Qing tea by high-performance liquid chromatography. The optimum conditions for micelle extraction were obtained as follows—15% (w/v) Genapol X-080 as extractant, pH 3.5, liquid/solid ratio 80, and extraction time, 40 min. For cloud point preconcentration, 20% (w/v) NaCl was added, and the solution was incubated at 55 °C for 30 min. The detection limits for the five anthraquinone derivatives were in the range of 0.55–3.30 ng ml−1. Average recoveries for the anthraquinone derivatives at three spiked levels were in the range of 84.3–104.1%. Relative standard deviations for six replicate determinations of Chang-Qing tea sample were below 2.39. The established method has been successfully applied to the determination of anthraquinone derivatives in Chang-Qing tea products from three different manufacturers.
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References
Akinlua A, Jochmann MA, Laaks J, Ewert A, Schmidt TC (2011) Microwave-assisted nonionic surfactant extraction of aliphatic hydrocarbons from petroleum source rock. Anal Chim Acta 691:48
Chen JK, Chen TT (2001) Liver-calming and wind-extinguishing herbs, Chinese medical herbology and pharmacology. Art of Medicine Press Inc, City of Industry, p 803
Drever BD, Anderson WG, Riedel G, Kim DH, Ryu JH, Choi DY, Platt B (2008) The seed extract of Cassia obtusifolia offers neuroprotection to mouse hippocampal cultures. J Pharmacol Sci 107:380
Ferrera ZS, Sanz CP, Santana CM, Rodriguez JJS (2004) The use of micellar systems in the extraction and pre-concentration of organic pollutants in environmental samples. Trends Anal Chem 23:469
Jin DH, Liang FH, Li JY (2007) Microwave assisted and liquid chromatography for simultaneous determination of anthraquinone derivatives in semen cassiae. J Mol Sci 23:429
Ju MS, Kim HG, Choi JG, Ryu JH, Hur J, Kim YJ, Oh MS (2010) Cassiae semen, a seed of Cassia obtusifolia, has neuroprotective effects in Parkinson’s disease models. Food Chem Toxicol 48:2037
Li HB, Fang KY, Li XE (2007) Study on determination methods of anthraquinones component of Cassia tora L. China Food Sci 28:427
Li GL, Xiao YQ, Zhang C, Li L, Pang Z (2009) Comparison study of two types of constituents in seeds of Cassia obtusifolia before and after roasted. China J Chin Materia Medica 34:1364
Ortega AC, da Silva DC, Visentainer JV, de Souza NE, Almeida VD, Oliveira CC (2011) Determination of vitamins A and E exploiting cloud point extraction and micellar liquid chromatography. Anal Lett 44:778
Ou M (1992) Chinese–English manual of common-used in traditional Chinese medicine. Guangdong Science and Technology Publishing House, Guangdong, p 243
Pourreza N, Rastegarzadeh S, Larki A (2011) Determination of allura red in food samples after cloud point extraction using mixed micelles. Food Chem 126:1465
Tang T, Qian K, Shi TY, Wang F, Li JQ, Cao YS (2010) Determination of triazole fungicides in environmental water samples by high performance liquid chromatography with cloud point extraction using polyethylene glycol 600 monooleate. Anal Chim Acta 680:26
The Pharmacopoeia Committee of China (2010) The Chinese pharmacopoeia, part I. The Chemical Industry Publishing House, Beijing, p 135
Trivedi P, Kumar JK, Negi AS, Shanker K (2011) HPLC method development and validation of cytotoxic agent phenyl-heptatriyne in Bidens pilosa with ultrasonic-assisted cloud point extraction and preconcentration. Biomed Chromatogr 25:697
Wong SM, Wong MM, Seligmann O (1981) Anthraquinone glycosides from the seeds of Cassia tora. Phytochem 20:1951
Zhang WJ, Duan CM, Wang ML (2011) Analysis of seven sulphonamides in milk by cloud point extraction and high performance liquid chromatography. Food Chem 126:779
Zheng WJ, Wang SF, Chen XG, Hu ZD (2004) Identification and determination of active anthraquinones in Chinese teas by micellar electrokinetic capillary chromatography. Biomed Chromatogr 18:167
Acknowledgments
Financial support from the National Natural Science Foundation of China (20875020, 20575016) and the Natural Science Foundation of Hebei Province China (B2006000953) are gratefully acknowledged. The authors also thank the financial support from “The Introduction of Talents” program of Hebei University.
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Shi, Z., Jiang, H., Yan, J. et al. Determination of Anthraquinone Derivatives in Chang-Qing Tea by Using Cloud-Point Extraction and High-Performance Liquid Chromatography. Food Anal. Methods 5, 659–663 (2012). https://doi.org/10.1007/s12161-011-9299-9
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DOI: https://doi.org/10.1007/s12161-011-9299-9