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Validation of Two Variations of the QuEChERS Method for the Determination of Multiclass Pesticide Residues in Cereal-Based Infant Foods by LC–MS/MS

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Abstract

Over the past years to ensure food safety and particular for food that intend to be consumed by infants and young children, the European Union has adopted specific legislation concerning the control of pesticide residue levels in that kind of food. In this paper, a liquid chromatography tandem quadrupole mass spectrometry (LC–MS/MS) multiresidue method for the simultaneous analysis of 23 pesticides and metabolites chosen according to the Commission Directives 2006/141/EC, 2006/125/EC, and 96 multiclass pesticides and metabolites chosen according to their physicochemical properties is presented and validated. The extraction procedure is based on three modifications of the quick, easy, cheap, effective, rugged, and safe method according to the analyte. The analytical performance was demonstrated by the analysis of extracts from cereal-based infant foods, spiked at two concentration levels for each pesticide or metabolite. Good sensitivity and selectivity of the method were obtained with limits of quantification at 10 or 3 μg/kg, depending on the analyte. All pesticides and metabolites, except six cases, gave recoveries in the range of 60.4–125.4%, with relative standard deviations less than 29.7%, for both validation levels.

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References

  • Anastassiades M (2009) QuEChERS—a mini-multiresidue method for the analysis of pesticide residues in low-fat products. Available at http://www.quechers.com. Accessed 22 Mar 2009

  • Anastassiades M, Lehotay SJ, Stajnbaher D, Schenck FJ (2003) J Assoc Off Anal Chem Int 86:412–431

    CAS  Google Scholar 

  • Anastassiades M, Tasdelen B, Scherbaum E, Stajnbaher D (2007) Recent developments in QuEChERS methodology for pesticide multiresidue analysis. In: Ohkawa H, Miyagawa H, Lee P (eds) Pesticide chemistry: crop protection, public health, environmental safety. WILEY-VCH, Weinheim, pp 439–458. ISBN 978-3-527-31663-2

    Chapter  Google Scholar 

  • Barcelo D, Fernandez-Alba A (2005) Comprehensive analytical chemistry: chromatographic–mass spectrometric food analysis for trace determination of pesticide residues, volume XLIII. Wilson & Wilson’s, Amsterdam

    Google Scholar 

  • Cajka T, Hajslova J (2004) J Chromatogr A 1058:251–261

    CAS  Google Scholar 

  • CRL for Single Residue Methods (2007) Analysis of acidic pesticides in wheat flour samples by LC–MS(/MS) using the QuEChERS method (including optional alkaline hydrolysis to release covalently bound compounds). Available at: http://www.crl-pesticides.eu/library/docs/cf/acidicpesticides_wheat_quechers.pdf. Accessed 2 Jun 2009

  • EURACHEM/CITAC Guide (2000) Quantifying uncertainty in analytical measurement, 2nd edn. EURACHEM, Lisbon, pp 106–107

    Google Scholar 

  • European Commission (2006a) Council Directive 2006/125/EC of 5 December 2006 on processed cereal-based foods and baby foods for infants and young children. Off J Eur Union L 339:16–35, 6 Dec 2006

    Google Scholar 

  • European Commission (2006b) Council Directive 2006/141/EC of 22 December 2006 on infant formulae and follow-on formulae and amending Directive 1999/21/EC. Off J Eur Union L 401:1–33, 30 Dec 2006

    Google Scholar 

  • European Commission (2009) Document No. SANCO/10684/2009, method validation and quality control procedures for pesticide residues analysis in food and feed. Available at: http://ec.europa.eu/food/plant/protection/resources/qualcontrol_en.pdf. Accessed 11 Jul 2010

  • Federal Institutor of Risk Assessment (2009) List of pesticides for which methodological data is available at present. Available at: www.bfr.bund.de/cm/289/list_of_pesticides_for_which_methodological_data_ist_available_at_present.pdf. Accessed 26 Jan 2009

  • Hercegova A, Domotorova M, Matisova E (2007) J Chromatogr A 1153:54–73

    Article  CAS  Google Scholar 

  • Hernandez F, Pozo OJ, Sancho JV, Bijlsma L, Barreda M, Pitarch E (2006) J Chromatogr A 1109:242–252

    Article  CAS  Google Scholar 

  • Patel K, Fussell RJ, Goodall DM, Keely BJ (2004) Food Addit Contam: Part A Chem Anal Control Expo Risk Assess 21:658

    CAS  Google Scholar 

  • Stepan R, Ticha NJ, Hajslova J, Kovalczuk T, Kocourek V (2005) Food Addit Contam: Part A Chem Anal Control Expo Risk Assess 22:1231–1242

    CAS  Google Scholar 

  • Wang J, Cheung W, Grant D (2005) J Agric Food Chem 53:528

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Laboratory of Pesticide Residues, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, 145 61, Kifissia, Athens, Greece

    Chris J. Anagnostopoulos, Pipina Aplada Sarli, Konstantinos Liapis & George E. Miliadis

  2. Laboratory of General Chemistry, Science Department, Agricultural University of Athens, 118 55, Athens, Greece

    Serko A. Haroutounian

Authors
  1. Chris J. Anagnostopoulos
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  2. Pipina Aplada Sarli
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  3. Konstantinos Liapis
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  4. Serko A. Haroutounian
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  5. George E. Miliadis
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Correspondence to Chris J. Anagnostopoulos.

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Anagnostopoulos, C.J., Aplada Sarli, P., Liapis, K. et al. Validation of Two Variations of the QuEChERS Method for the Determination of Multiclass Pesticide Residues in Cereal-Based Infant Foods by LC–MS/MS. Food Anal. Methods 5, 664–683 (2012). https://doi.org/10.1007/s12161-011-9296-z

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  • DOI: https://doi.org/10.1007/s12161-011-9296-z

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