Abstract
A method using distillation–solid-phase extraction coupled with gas chromatography and flame ionization detector was developed for assaying the major volatile organic compounds (VOCs) (i.e., 3-methyl-1-butanol, 2-methyl-1-butanol, and 2-phenylethanol) in the Tuber melanosporum fermentation system. Satisfied linearity, precision, accuracy, and recovery obtained in the method validation test indicate that the method was simple, accurate, and reproducible. Furthermore, the developed analytical method was used for the dynamic monitoring of the T. melanosporum fermentation process, and the highest amount of major VOCs was occurred on days 9 and 10. This method can be employed to monitor the VOCs in the fermentation process and thus to evaluate the VOC quality of the truffle fermentation system.
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Acknowledgements
Financial support from the National Natural Science Foundation of China (NSFC, Project Nos. 20976038 and 21176059), the Key Project of Chinese Ministry of Education (Project No. 210132), Hubei Provincial Natural Science Foundation for Innovative Research Team (Project No. 2008CDA002), Scientific Research Key Project of Hubei Provincial Department of Education (Project No. Z20101401), Discipline Leader Project of Wuhan Municipality (Project No. 200951830553), Key Technology R&D Program of Wuhan Municipality (Project No. 201120822280-2), the Open Project Programs for the Key Laboratory of Fermentation Engineering (Ministry of Education), the National Key Laboratory of Biochemical Engineering (Project No. 2010KF-06), and the State Key Laboratory of Bioreactor Engineering are gratefully acknowledged.
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Tang, YJ., Wang, G., Li, YY. et al. Quantitative Determination for the Major Volatile Organic Compounds of Tuber melanosporum Fermentation System by Distillation–Solid-Phase Extraction–Gas Chromatography. Food Anal. Methods 5, 651–658 (2012). https://doi.org/10.1007/s12161-011-9293-2
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DOI: https://doi.org/10.1007/s12161-011-9293-2