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Development of a DNA Array for the Simultaneous Detection and Identification of Sugar Thick Juice Bacterial Contaminants

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Abstract

Despite the use of generally accepted good storage practices, sugar thick juice degradation caused by microbiological contamination occasionally occurs, causing considerable financial loss. In this study, a DNA array was developed for simultaneous detection and identification of the most prominent microflora present during thick juice storage, which may cause degradation of the thick juice. Specific oligonucleotides were developed for several bacterial taxa, including the genera Bacillus, Kocuria, Staphylococcus and Tetragenococcus and the species Aerococcus viridans, Leuconostoc mesenteroides and Tetragenococcus halophilus. The DNA array was validated using both pure cultures and industrial samples. In addition, comparisons were made between the developed array, PCR assays specifically targeting the thick juice contaminants and classical microbial platings. The array was found to be reliable and sensitive enough to detect and identify the target bacteria. In addition, the array was used to monitor the target microbial populations in thick juice during long-term storage and degradation. Results are discussed in relation to DNA stability in thick juice.

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Acknowledgments

We are grateful to the Südzucker company and the Lessius University College, Campus De Nayer for financing the PhD of A. Justé, and to the Tiense Suikerraffinaderij (Tienen, Belgium) and Südzucker (Germany) for supplying the thick juice and their committed cooperation. In addition, the authors thank Michael Waud for editing the manuscript.

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Authors and Affiliations

  1. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), Consortium for Industrial Microbiology and Biotechnology (CIMB), Department of Microbial and Molecular Systems (M2S), KULeuven Association, Lessius University College, De Nayer Campus, B-2860, Sint-Katelijne-Waver, Belgium

    Annelies Justé, Bart Lievens, Ingeborg Frans & Kris A. Willems

  2. Scientia Terrae Research Institute, B-2860, Sint-Katelijne-Waver, Belgium

    Annelies Justé, Bart Lievens, Ingeborg Frans & Kris A. Willems

  3. Leuven Food Science and Nutrition Research Centre (LFoRCe), B-3001, Leuven, Belgium

    Annelies Justé, Bart Lievens, Ingeborg Frans, Chris W. Michiels & Kris A. Willems

  4. Südzucker AG, Mannheim/Ochsenfurt, ZAFES, 67283, Obrigheim/Pfalz, Germany

    Michael Klingeberg

  5. Center for Food and Microbial Technology, Department of Microbial and Molecular Systems, KULeuven, B-3001, Leuven, Belgium

    Chris W. Michiels

Authors
  1. Annelies Justé
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  2. Bart Lievens
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  3. Ingeborg Frans
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  4. Michael Klingeberg
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  5. Chris W. Michiels
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  6. Kris A. Willems
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Corresponding author

Correspondence to Bart Lievens.

Additional information

A. Justé and B. Lievens equally contributed to this work

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Justé, A., Lievens, B., Frans, I. et al. Development of a DNA Array for the Simultaneous Detection and Identification of Sugar Thick Juice Bacterial Contaminants. Food Anal. Methods 4, 173–185 (2011). https://doi.org/10.1007/s12161-010-9152-6

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  • DOI: https://doi.org/10.1007/s12161-010-9152-6

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