Development of an ELISA Reverse-Based Assay to Assess the Presence of Mycotoxins in Cereal Flour


The first successful application of the ELISA reverse method and device (ER m&d), in the context of food safety and traceability, was developed in our laboratories to detect and quantify CP4EPSPS and Cry1AB genetically modified related proteins in soy and maize samples, respectively. To prove the versatility and the transferability of the technology, the ER was here applied to assess the presence of mycotoxins in cereals. Appropriate protocols were developed to assess the presence of deoxynivalenol and ochratoxin A and the ER was tested on contaminated wheat samples. In particular, deoxynivalenol (DON) and ochratoxin A (OTA) were detected in a range of values from 430 to 5,000 µg kg−1 and from 1.7 to 10 µg kg−1, respectively. The assays were optimized to reach a limit of quantification equal to 550 and 1.8 µg kg−1 for DON and OTA, respectively.

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The on-going collaboration between DG-Joint Research Centre (JRC) and University of Molise was enhanced by the ENEA-Innovation Relay Centre IRIDE as part of the IRC NETWORK created by the European Commission. Authors wish to acknowledge the colleagues of IRMM DG-JRC in Geel (Belgium) for valuable discussions as Community Reference Laboratory (CRL) for mycotoxins by Regulation 2006/776/EC.

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Correspondence to Monica Ermolli.

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Folloni, S., Bellocchi, G., Kagkli, DM. et al. Development of an ELISA Reverse-Based Assay to Assess the Presence of Mycotoxins in Cereal Flour. Food Anal. Methods 4, 221–227 (2011).

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  • ELISA Reverse
  • Deoxynivalenol
  • Ochratoxin A
  • Food Safety