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Quantitative Determination of Deoxynivalenol (DON) Using the Amplified Luminescent Proximity Homogeneous Assay (AlphaLISA)

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Abstract

An indirect competitive Amplified Luminescent Proximity Homogeneous Assay (AlphaLISA) was established by using anti-deoxynivalenol (DON) polyclonal antibody and coating antigen DON-bovine serum albumin (BSA) for detection of DON in cereals. DON-BSA was coated on Acceptor bead, the kit also contains donor bead sensitizer particles coated with streptomycin. The optional test conditions and analytical performance of the method were studied. Working concentration ranged from 0.007 to 100 ng/ml and the sensitivity for detection was 0.007 ng/ml. The intra- and inter-batch coefficient of variation (CV) of the assay were both below 5%. The recovery rate of artificially contaminated cereals ranged from 81.1% to 110.9% while the mean recovery of DON from cereals was 94.4%. This study suggests that DON-AlphaLISA method is a good method with high sensitivity and rapidity for quantitative analyzing DON in cereals.

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Acknowledgment

This work was financially supported by the Hi-Tech program, Ministry of Science and Technology of China (No.2008AA10Z415) and the State Key Laboratory of Food Science and Technology, Jiangnan University (No.SKLF-MB-200801).

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Authors and Affiliations

  1. Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, 214063, China

    Zhou Bin, Wang Ke & Huang Biao

  2. School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu, 214063, China

    Jin Jian & Chen Yun

  3. Shanghai Boyang Biotechnology Company Ltd, Shanghai, 210000, China

    Zhao Weiguo

Authors
  1. Zhou Bin
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  2. Wang Ke
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  3. Jin Jian
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  4. Chen Yun
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  5. Huang Biao
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  6. Zhao Weiguo
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Correspondence to Huang Biao.

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Bin, Z., Ke, W., Jian, J. et al. Quantitative Determination of Deoxynivalenol (DON) Using the Amplified Luminescent Proximity Homogeneous Assay (AlphaLISA). Food Anal. Methods 4, 228–232 (2011). https://doi.org/10.1007/s12161-010-9148-2

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  • DOI: https://doi.org/10.1007/s12161-010-9148-2

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