Abstract
Dot-immunogold filtration assay (DIGFA) is a rapid and simple immunoassay for qualitative screening of analytes, but now its application is often restricted by relatively low sensitivity. Here, a secondary antibody-based method was developed to activate nitrocellulose membranes and therefore improve the sensitivity of DIGFA. With such activated membranes, the detection sensitivity for enrofloxacin was improved approximately five times in pure matrix. The same enhancement of DIGFA was also obtained with food samples. These results allowed us to suggest a promising application of the established technique for development of more sensitive DIGFA for low molecular contaminants in food.
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Sui, J., Lin, H., Xu, Y. et al. Enhancement of Dot-Immunogold Filtration Assay (DIGFA) by Activation of Nitrocellulose Membranes with Secondary Antibody. Food Anal. Methods 4, 245–250 (2011). https://doi.org/10.1007/s12161-010-9137-5
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DOI: https://doi.org/10.1007/s12161-010-9137-5