Food Analytical Methods

, Volume 3, Issue 4, pp 313–320 | Cite as

Visual and Rapid Detection of Two Genetically Modified Soybean Events Using Loop-mediated Isothermal Amplification Method

Article

Abstract

To develop effective alternatives for detecting genetically modified organisms (GMOs), we reported one optimized visual loop-mediated isothermal amplification (LAMP) method for the detection of exogenous DNA targets from two GM soybean events in this study. This isothermal amplification can be performed within 40 min without polymerase chain reaction (PCR) equipment and the derived LAMP products can be directly observed by naked eye employing SybrGreen I dye instead of conventional gel electrophoresis analysis. The limits of detection of these established visual LAMP assays were about 4 copies of haploid soybean genomic DNA, and which were much higher than those of reported conventional PCR assays. Furthermore, the high specificity of LAMP assays was determined. All the results demonstrated that the developed visual LAMP assays are convenient, cost-efficient, and rapid for on spot detection of GMOs.

Keywords

Loop-mediated Isothermal Amplification Visual Detection Genetically Modified Soybean 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.GMO Detection Laboratory, SJTU-Bor Luh Food Safety Center, School of life Science and BiotechnologyShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China
  2. 2.Development Center for Science and TechnologyMinistry of AgricultureBeijing100026China
  3. 3.Bio-X Research Center, Key Laboratory of Genetics and Development and Neuropsychiatric Diseases, Ministry of EducationShanghai Jiao Tong UniversityShanghai200240People’s Republic of China

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