Assays of the Amino Acid Tryptophan in Cherries by HPLC-Fluorescence
- 372 Downloads
Seven varieties of cherry from the “Valle del Jerte” in the Spanish Region of Extremadura were analyzed quantitatively and qualitatively for their content of the amino acid tryptophan. Tryptophan is an essential amino acid in humans, being the precursor of the indolamines serotonin and melatonin which are biogenic amines with great importance in human sleep function. It is also of great importance in the synthesis of indole alkaloids in fruit. The objective of the present work was to determine the levels of tryptophan in “Valle del Jerte” (Extremadura, Spain) cherry varieties by high-performance liquid chromatography with fluorescence detection. Tryptophan was found in all seven varieties tested, the mean concentration being 54.48 ± 17.14 g/ml. There were major differences between the varieties. The highest levels corresponded to the variety Navalinda and the lowest to Pico colorado. The tryptophan content of all the cherry varieties tested, in particular Navalinda, will have importance as the precursor for the biosynthesis of the biogenic amines.
KeywordsTryptophan Prunus avium HPLC-FL Cherry
The authors gratefully acknowledge the funding for this research to Convention Uex-Agrupación de Cooperativas Valle del Jerte 067/07 and PDT08A008 Junta de Extremadura and the technical assistance of Ms Elena Circujano Vadillo, our laboratory technician.
- Alonso J (2001) La calidad en el proceso de manipulación de la cereza en la Agrupación de Cooperativas del Valle del Jerte. Fruticultura Profesional 118:49–55Google Scholar
- Cubero J, Valero V, Sánchez J, Rivero M, Parvez H, Rodríguez AB, Barriga C (2005b) The circadian rhythm of tryptophan in breast milk affects the rhythms of 6sulfatoxymelatonin and sleep in newborn. Neuroendocrinol Lett 26:657–661Google Scholar
- Cubero J, Narciso D, Terrón P, Rial R, Esteban S, Rivero M, Parvez H, Rodríguez A, Barriga C (2007) Chrononutrition applied to formula milks consolidate infant’s sleep/wake cycle. Neuroendocrinol Lett 28:360–366Google Scholar
- Hajak G, Huether G, Blanke J, Freyer B, Poeggeler P, Reimer A, Rodenbeck A, Schulz-Varszegi A, Ruether M (1991) The influence of intravenous L-tryptophan on plasma melatonin and sleep in men. Pharmacopsychiat 24:17–21Google Scholar
- Hoenicke H, Simat JT, Steinhart H, Christoph N, Kohler HJ, Schwab A (1999) Determination of tryptophan and tryptophan metabolites in grape must and wine. Tryptophan, Serotonin and Melatonin; Basic Aspect and Applications. Kluwer Academic/Plenum Publisher. New York 86:671–677Google Scholar
- Reiter RJ, Tan D, Manchester LC, Simopoulus AP, Maldonado MD, Flores LJ, Terrón MP (2007) Melatonin in edible plants (phytomelatonin): Identification, concentration, bioavailability and proposed functions. World Rev Nutr Diet 97:211–230Google Scholar