Skip to main content

Advertisement

SpringerLink
Log in

Effect of Lactate Fermentation Type on the Biochemical Methane Potential of Tequila Vinasse

  • Published:
BioEnergy Research Aims and scope Submit manuscript

Abstract

In a two-stage anaerobic digestion (AD) process approach, the relation between the type of fermentation that occurs during the acidogenic stage and methane production potential from tequila vinasse (TV) remains unexplored. This study aimed to investigate the effect of lactate fermentation type on the biochemical methane potential (BMP) of TV. To this end, a series of batch cultures were carried out to perform two sequential lactate-type fermentations, i.e., first (carbohydrates → lactate + acetate) and second (lactate + acetate → butyrate + H2) fermentation, which were further tested for mesophilic BMP using an automatic gas measuring device. Raw TV was also included in the comparison as a control (single-stage AD). During the primary fermentation, 75% of the total fermentable sugars were metabolized into lactate (12.6 g/L) and acetate (6.6 g/L) by the bacterial cluster mainly encompassing Klebsiella, Enterobacter, and Streptococcus, as supported by Illumina MiSeq sequencing analysis. During the secondary fermentation, lactate and acetate were bioconverted into butyrate (5.1 g/L) and hydrogen (115.6 NmL/g VSadded) by Clostridium. During methanogenesis, all tested feedstocks resulted in different BMP performances. The primary lactate fermentation led to the highest BMP (435.9 NmL CH4/g VSadded), methane production rate (105.8 NmL CH4/day), and chemical oxygen demand removal efficiency (92.9%), which were 46.2%, 89.3%, and 8.8%, respectively, higher than those attained from raw TV. Methanobacterium and Methanolinea dominated in the starter granular sludge culture, suggesting the hydrogenotrophic pathway was key for methane formation. This study shows for the first time the viability of the lactate-type fermentation for designing enhanced two-stage TV-fed AD processes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. López-López A, Davila-Vazquez G, León-Becerril E, Villegas-García E, Gallardo-Valdez J (2010) Tequila vinasses: generation and full scale treatment processes. Rev Environ Sci Biotechnol 9:109–116. https://doi.org/10.1007/s11157-010-9204-9

    CAS  Google Scholar 

  2. Consejo regulador del Tequila (CRT). Available at: https://www.crt.org.mx/estadisticascrtweb/; 2018. Accessed  6 Jan 2020

  3. Buitron G, Carvajal C (2010) Biohydrogen production from tequila vinasses in an anaerobic sequencing batch reactor: effect of initial substrate concentration, temperature and hydraulic retention time. Bioresour Technol 01:9071–9077. https://doi.org/10.1016/j.biortech.2010.06.127

    CAS  Google Scholar 

  4. van Lier JB, Mahmoud N, Zeeman G (2008) Anaerobic wastewater treatment, in biological wastewater treatment: principles modelling and design. IWA Publishing, London, pp 401–442. https://doi.org/10.1021/es00154a002

    Google Scholar 

  5. Lindner J, Zielonka S, Oechsner H, Lemmer A (2016) Is the continuous two-stage anaerobic digestion process well suited for all substrates? Bioresour Technol 200:470–476. https://doi.org/10.1016/j.biortech.2015.10.052

    CAS  PubMed  Google Scholar 

  6. Ruggeri B, Tommasi T, Sanfilippo S (2015) Two-step anaerobic digestion process, in BioH2 & BioCH4 through anaerobic digestion. Springer-Verlag:161–191. https://doi.org/10.1007/978-1-4471-6431-9

    Google Scholar 

  7. Pohland FG, Ghosh S (1971) Developments in anaerobic stabilization of organic wastes: the two-phase concept. Environ Lett 1:255–266. https://doi.org/10.1080/00139307109434990

    CAS  PubMed  Google Scholar 

  8. Kleerebezem R, Joosse B, Rozendal R, Van Loosdrecht MCM (2015) Anaerobic digestion without biogas? Rev Environ Sci Biotechnol 14:787–801. https://doi.org/10.1007/s11157-015-9374-6

    CAS  Google Scholar 

  9. Venkata Mohan S, Nikhil GN, Chiranjeevi P, Nagendranatha Reddy C, Rohit MV, Naresh Kumar A, Sarkar O (2016) Waste biorefinery models towards sustainable circular bioeconomy: critical review and future perspectives. Bioresour Technol 215:2–12. https://doi.org/10.1016/j.biortech.2016.03.130

    CAS  PubMed  Google Scholar 

  10. Sträuber H, Lucas R, Kleinsteuber S (2016) Metabolic and microbial community dynamics during the anaerobic digestion of maize silage in a two-phase process. Appl Microbiol Biotechnol 100:479–491. https://doi.org/10.1007/s00253-015-6996-0

    CAS  PubMed  Google Scholar 

  11. Feng K, Deng Z, Wang Q, Zhang Y, Zheng C (2020) Effect of pre-fermentation types on the potential of methane production and energy recovery from food waste. Renew Energy 146:1588–1595. https://doi.org/10.1016/j.renene.2019.07.127

    CAS  Google Scholar 

  12. Chen X, Yuan H, Zou D, Liu Y, Zhu B, Chufo A, Jaffar M, Li X (2015) Improving biomethane yield by controlling fermentation type ofacidogenic phase in two-phase anaerobic co-digestion of food wasteand rice straw. Chem Eng J 273:254–260. https://doi.org/10.1016/j.cej.2015.03.067

    CAS  Google Scholar 

  13. Zhou M, Yan B, Wong JWC, Zhang Y (2018) Enhanced volatile fatty acids production from anaerobic fermentation offood waste: a mini-review focusing on acidogenic metabolic pathways. Bioresour Technol 248:68–78. https://doi.org/10.1016/j.biortech.2017.06.121

    CAS  PubMed  Google Scholar 

  14. Pipyn P, Verstraete W (1981) Lactate and ethanol as intermediates in two-phase anaerobic digestion. Biotechnol Bioeng 23:1145–1154. https://doi.org/10.1002/bit.260230521

    CAS  Google Scholar 

  15. Detman A, Mielecki D, Pleśniak Ł, Bucha M, Janiga M, Matyasik I, Chojnacka A, Jędrysek MO, Błaszczyk MK, Sikora A (2018) Methane-yielding microbial communities processing lactate-rich substrates: a piece of the anaerobic digestion puzzle. Biotechnol Biofuels 11:116. https://doi.org/10.1186/s13068-018-1106-z

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Wu Y, Wang C, Liu X, Ma H, Wu J, Zuo J, Wang K (2016) A new method of two-phase anaerobic digestion for fruit and vegetable waste treatment. Bioresour Technol 211:16–23. https://doi.org/10.1016/j.biortech.2016.03.050

    CAS  PubMed  Google Scholar 

  17. Satpathy P, Steinigeweg S, Siefert E, Cypionka H (2017) Effect of lactate and starter inoculum on biogas production from fresh maize and maize silage. Adv Microbiol 7:358–376. https://doi.org/10.4236/aim.2017.75030

    CAS  Google Scholar 

  18. Garcia-Aguirre J, Aymerich E, González-Mtnez de Goñi J, Esteban-Gutierrez M (2017) Selective VFA production potential from organic waste streams: assessing temperature and pH influence. Bioresour Technol 244:1081–1088. https://doi.org/10.1016/j.biortech.2017.07.187

    CAS  PubMed  Google Scholar 

  19. García-Depraect O, Gómez-Romero J, León-Becerril E, López-López A (2017) A novel biohydrogen production process: co-digestion of vinasse and Nejayote as complex raw substrates using a robust inoculum. Int J Hydrog Energy 42:5820–5831. https://doi.org/10.1016/j.ijhydene.2016.11.204

    CAS  Google Scholar 

  20. García-Depraect O, Valdez-Vázquez I, Rene ER, Gómez-Romero J, López-López A, León-Becerril E (2019) Lactate and acetate based biohydrogen production through dark co- fermentation of tequila vinasse and nixtamalization wastewater: metabolic and microbial community dynamics. Bioresour Technol 282:236–244. https://doi.org/10.1016/j.biortech.2019.02.100

    CAS  PubMed  Google Scholar 

  21. García-Depraect O, Rene ER, Diaz-Cruces VF, León-Becerril E (2019) Effect of process parameters on enhanced biohydrogen production from tequila vinasse via the lactate-acetate pathway. Bioresour Technol 273:618–626. https://doi.org/10.1016/j.biortech.2018.11.056

    CAS  PubMed  Google Scholar 

  22. García-Depraect O, León-Becerril E (2018) Fermentative biohydrogen production from tequila vinasse via the lactate-acetate pathway: operational performance, kinetic analysis and microbial ecology. Fuel 234:151–160. https://doi.org/10.1016/j.fuel.2018.06.126

    CAS  Google Scholar 

  23. García-Depraect O, Rene ER, Gómez-Romero J, López-lópez A, León-Becerril E (2019) Enhanced biohydrogen production from the dark co-fermentation of tequila vinasse and nixtamalization wastewater: novel insights into ecological regulation by pH. Fuel 253:159–166. https://doi.org/10.1016/j.fuel.2019.04.147

    CAS  Google Scholar 

  24. Fuess LT, Ferraz ADN, Machado CB, Zaiat M (2018) Temporal dynamics and metabolic correlation between lactate-producing and hydrogen-producing bacteria in sugarcane vinasse dark fermentation: the key role of lactate. Bioresour Technol 247:426–433. https://doi.org/10.1016/j.biortech.2017.09.121

    CAS  PubMed  Google Scholar 

  25. Fuess LT, Zaiat M, Augusto Oller do Nascimento C (2019) Novel insights on the versatility of biohydrogen production from sugarcane vinasse via thermophilic dark fermentation: impacts of pH-driven operating strategies on acidogenesis metabolite profiles. Bioresour Technol 286:121379. https://doi.org/10.1016/j.biortech.2019.121379

    CAS  PubMed  Google Scholar 

  26. Asunis F, De Gioannis G, Isipato M, Muntoni A, Polettini A, Pomi R, Rossi A, Spiga D (2019) Control of fermentation duration and pH to orient biochemicals and biofuels production from cheese whey. Bioresour Technol 289:121722. https://doi.org/10.1016/j.biortech.2019.121722

    CAS  PubMed  Google Scholar 

  27. Blanco VMC, Oliveira GHD, Zaiat M (2019) Dark fermentative biohydrogen production from synthetic cheese whey in an anaerobic structured-bed reactor: performance evaluation and kinetic modeling. Renew Energy 139:1310–1319. https://doi.org/10.1016/j.renene.2019.03.029

    CAS  Google Scholar 

  28. Holliger C, Alves M, Andrade D, Angelidaki I, Astals S, Baier U, Bougrier C, Buffière P, Carballa M, de Wilde V, Ebertseder F, Fernández B, Ficara E, Fotidis I, Frigon JC, de Laclos HF, Ghasimi DS, Hack G, Hartel M, Heerenklage J, Horvath IS, Jenicek P, Koch K, Krautwald J, Lizasoain J, Liu J, Mosberger L, Nistor M, Oechsner H, Oliveira JV, Paterson M, Pauss A, Pommier S, Porqueddu I, Raposo F, Ribeiro T, Rüsch Pfund F, Strömberg S, Torrijos M, van Eekert M, van Lier J, Wedwitschka H, Wierinck I (2016) Towards a standardization of biomethane potential tests. Water Sci Technol 74:2515–2522. https://doi.org/10.2166/wst.2016.336

    CAS  PubMed  Google Scholar 

  29. Angelidaki I, Alves M, Bolzonella D, Borzacconi L, Campos JL, Guwy AJ, Kalyuzhnyi S, Jenicek P, van Lier J (2009) Defining the biomethane potential (BMP) of solid organic wastes and energy crops: a proposed protocol for batch assays. Water Sci Technol 59:927–934. https://doi.org/10.2166/wst.2009.040

    CAS  PubMed  Google Scholar 

  30. APHA (2005) Standard methods for the examination of water and wastewater, 21st edn. American Public Health Association/American Water Works Association/Water Environmental Federation, Washington DC

    Google Scholar 

  31. RTLGenomics, Data analysis methodology. http://www.rtlgenomics.com/docs/Data_Analysis_Methodology.pdf. Accessed 15 Oct 2018

  32. Strömberg S, Nistor M, Liu J (2014) Towards eliminating systematic errors caused by the experimental conditions in biochemical methane potential (BMP) tests. Waste Manag 34:1939–1948. https://doi.org/10.1016/j.wasman.2014.07.018

    PubMed  Google Scholar 

  33. Moset V, Al-zohairi N, Møller HB (2015) The impact of inoculum source, inoculum to substrate ratio and sample preservation on methane potential from different substrates. Biomass Bioenergy 83:474–482. https://doi.org/10.1016/j.biombioe.2015.10.018

    CAS  Google Scholar 

  34. Schievano A, Tenca A, Lonati S, Manzini E, Adani F (2014) Can two-stage instead of one-stage anaerobic digestion really increase energy recovery from biomass? Appl Energy 124:335–342. https://doi.org/10.1016/j.apenergy.2014.03.024

    CAS  Google Scholar 

  35. Tao Y, Hu X, Zhu X, Jin H, Xu Z, Tang Q, Li X (2016) Production of butyrate from lactate by a newly isolated Clostridium sp. BPY5. Appl Biochem Biotechnol 179:361–374. https://doi.org/10.1007/s12010-016-1999-6

    CAS  PubMed  Google Scholar 

  36. Cabrol L, Marone A, Tapia-Venegas E, Steyer JP, Ruiz-Filippi G, Trably E (2017) Microbial ecology of fermentative hydrogen producing bioprocesses: useful insights for driving the ecosystem function. FEMS Microbiol Rev 41:158–181. https://doi.org/10.1093/femsre/fuw043

    CAS  PubMed  Google Scholar 

  37. Schwalm ND III, Mojadedi W, Gerlach ES, Benyamin M, Perisin MA, Akingbade KL (2019) Developing a microbial consortium for enhanced metabolite production from simulated food waste. Fermentation 5(4):98. https://doi.org/10.3390/fermentation5040098

    Google Scholar 

  38. Hung C, Cheng C, Guan D, Wang S (2010) Interactions between Clostridium sp. and other facultative anaerobes in a self-formed granular sludge hydrogen-producing bioreactor. Bioresour Technol 6:2–9. https://doi.org/10.1016/j.ijhydene.2010.06.010

    CAS  Google Scholar 

  39. Hung CH, Cheng CH, Guan DW, Wang ST, Hsu SC, Liang CM (2011) Interactions between Clostridium sp. and other facultative anaerobes in a self-formed granular sludge hydrogen-producing bioreactor. Int J Hydrog Energy 36:8704–8711. https://doi.org/10.1016/j.ijhydene.2010.06.010

    CAS  Google Scholar 

  40. Shah FA, Mahmood Q, Shah MM, Pervez A, Asad SA (2014) Microbial ecology of anaerobic digesters: the key players of anaerobiosis. Sci World J 21. https://doi.org/10.1155/2014/183752

    Google Scholar 

  41. Pol LWH, Lopes SIDC, Lettinga G, Lens PNL (2004) Anaerobic sludge granulation. Water Res 38:1376–1389. https://doi.org/10.1016/j.watres.2003.12.002

    CAS  Google Scholar 

  42. Toledo-Cervantes A, Guevara-Santos N, Arreola-Vargas J, Snell-Castro R, Méndez-Acosta HO (2018) Performance and microbial dynamics in packed-bed reactors during the long-term two-stage anaerobic treatment of tequila vinasses. Biochem Eng J 138:12–20. https://doi.org/10.1016/j.bej.2018.06.020

    CAS  Google Scholar 

  43. Arreola-Vargas J, Snell-Castro R, Rojo-Liera NM, González-Álvarez V, Méndez-Acosta HO (2018) Effect of the organic loading rate on the performance and microbial populations during the anaerobic treatment of tequila vinasses in a pilot-scale packed bed reactor. J Chem Technol Biotechnol 93:591–599. https://doi.org/10.1002/jctb.5413

    CAS  Google Scholar 

Download references

Acknowledgments

Víctor F. Diaz-Cruces acknowledges CONACYT for the MSc scholarship 621447.

Funding

This work was financially supported by Fondo SENER- CONACYT Sustentabilidad Energética, Clúster Biocombustibles Gaseosos (project 247006).

Author information

Authors and Affiliations

  1. Environmental Technology Department, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C, Av. Normalistas 800, Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico

    Víctor F. Diaz-Cruces, Octavio García-Depraect & Elizabeth León-Becerril

Authors
  1. Víctor F. Diaz-Cruces
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Octavio García-Depraect
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Elizabeth León-Becerril
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Elizabeth León-Becerril.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Diaz-Cruces, V.F., García-Depraect, O. & León-Becerril, E. Effect of Lactate Fermentation Type on the Biochemical Methane Potential of Tequila Vinasse. Bioenerg. Res. 13, 571–580 (2020). https://doi.org/10.1007/s12155-020-10093-z

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12155-020-10093-z

Keywords

Search

Navigation