Abstract
Enzymatic hydrolysis accounts for 20% of the total cost in the conversion process of lignocellulosic biomass into bioethanol. Therefore, production of biomass-degrading enzymes by using lignocellulosic residue as a fermentation substrate may be an alternative to decrease the production costs. In this study, corncob (CC) has been pretreated by liquid hot water (LHW) at 200 °C for 30 min and used as inducer source for production of biomass-degrading enzymes by Trichoderma reesei MUM 97.53. The pretreatment was used to increase the cellulose content and the accessibility to lignocellulosic material. Although the filamentous fungus secreted a broad range of cellulolytic and hemicellulolytic enzymes when grown on untreated CC, higher enzyme productions were obtained when cultured on LHW-pretreated CC in a 2-L stirred tank bioreactor (STB). Besides, the effects of aeration (2 and 4 vvm) and agitation (150 and 250 rpm) rates on enzyme production were studied by submerged fermentation in a batch STB and correlated with the volumetric oxygen transfer coefficient (kLa). Maximal cellulase, xylanase, and β-xylosidase productions were found at 150 rpm and 4 vvm, while the highest β-glucosidase levels were obtained at 150 rpm and 2 vvm, that corresponded to kLa values of 32.50 h−1 and 16.41 h−1, respectively. At higher agitation, a lower enzymatic production was observed probably due to the high shear stress in the fungal hyphae.
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Acknowledgments
The authors thank Professor Dr. Nelson Lima from MUM (Micoteca da Universidade do Minho, Portugal) by the fungal strain.
Funding
Michele Michelin is a recipient of a FCT fellowship (SFRH/BPD/100786/2014). This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte.
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Michelin, M., Mota, A.M.O., Silva, D.P. et al. Production of Biomass-Degrading Enzymes by Trichoderma reesei Using Liquid Hot Water-Pretreated Corncob in Different Conditions of Oxygen Transfer. Bioenerg. Res. 12, 583–592 (2019). https://doi.org/10.1007/s12155-019-09991-8
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DOI: https://doi.org/10.1007/s12155-019-09991-8