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A Review of Holocellulase Production Using Pretreated Lignocellulosic Substrates

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Abstract

The economic viability of enzyme-based lignocellulosic biomass biorefineries depends on the low cost of holocellulose-degrading enzymes necessary for decomposing biomass into fermentable sugars and other value-added products. The high costs of commercial enzymes and the high enzyme loadings required for biomass hydrolysis motivates the use of lignocellulose as feedstock for on-site, integrated production of holocellulases in biorefineries. However, due to high recalcitrance, raw lignocellulose limits fungal growth and enzyme production. Pretreatment technologies can enhance enzyme production when employing lignocellulosic materials as substrate. This review provides a brief description of currently available pretreatment technologies and illustrates the potential of pretreating lignocellulosic wastes for enzyme production with filamentous fungi.

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Acknowledgments

The authors acknowledge the receipt of financial support from the Brazilian National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES), Foundation for Research Support of the Federal District (FAPDF), and the National Institute for Science and Technology of Bioethanol.

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  1. Laboratory of Enzymology, Department of Cell Biology, University of Brasília, DF, Brasília, 70910-900, Brazil

    Caio de Oliveira Gorgulho Silva & Edivaldo Ximenes Ferreira Filho

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  1. Caio de Oliveira Gorgulho Silva
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  2. Edivaldo Ximenes Ferreira Filho
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Correspondence to Caio de Oliveira Gorgulho Silva.

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de Oliveira Gorgulho Silva, C., Filho, E.X.F. A Review of Holocellulase Production Using Pretreated Lignocellulosic Substrates. Bioenerg. Res. 10, 592–602 (2017). https://doi.org/10.1007/s12155-017-9815-x

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