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BioEnergy Research

, Volume 9, Issue 4, pp 1204–1215 | Cite as

Anaerobic Co-digestion of Swine Manure and Microalgae Chlorella sp.: Experimental Studies and Energy Analysis

  • Meng Wang
  • Eunyoung Lee
  • Qiong Zhang
  • Sarina J. ErgasEmail author
Article

Abstract

Integration of algae production with livestock waste management has the potential to recover energy and nutrients from animal manure, while reducing discharges of organic matter, pathogens, and nutrients to the environment. In this study, microalgae Chlorella sp. were grown on centrate from anaerobically digested swine manure. The algae were harvested for mesophilic anaerobic digestion (AD) with swine manure for bioenergy production. Low biogas yields were observed in batch AD studies with algae alone, or when algae were co-digested with swine manure at ≥43 % algae (based on volatile solids [VS]). However, co-digestion of 6–16 % algae with swine manure produced similar biogas yields as digestion of swine manure alone. An average methane yield of 190 mL/g VSfed was achieved in long-term semi-continuous co-digestion studies with 10 ± 3 % algae with swine manure. Data from the experimental studies were used in an energy analysis assuming the process was scaled up to a concentrated animal feeding operation (CAFO) with 7000 pigs with integrated algae-based treatment of centrate and co-digestion of manure and the harvested algae. The average net energy production for the system was estimated at 1027 kWh per day. A mass balance indicated that 58 % of nitrogen (N) and 98 % of phosphorus (P) in the system were removed in the biosolids. A major advantage of the proposed process is the reduction in nutrient discharges compared with AD of swine waste without algae production.

Keywords

Microalgae Chlorella sp. Swine manure Anaerobic co-digestion CAFO Energy production 

Notes

Acknowledgments

The authors want to acknowledge the assistance of Merrill P. Dilbeck, an undergraduate student at the University of South Florida. This material is based upon work supported by the National Science Foundation under Grant No. 1243510. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation.

Supplementary material

12155_2016_9769_MOESM1_ESM.docx (410 kb)
ESM 1 (DOCX 409 kb)

References

  1. 1.
    Han MJ, Behera SK, Park HS (2012) Anaerobic co-digestion of food waste leachate and piggery wastewater for methane production: statistical optimization of key process parameters. J Chem Technol Biotechnol 87(11):1541–1550CrossRefGoogle Scholar
  2. 2.
    Bernet N, Béline F (2009) Challenges and innovations on biological treatment of livestock effluents. Bioresour Technol 100(22):5431–5436CrossRefPubMedGoogle Scholar
  3. 3.
    U.S. Environmental Protection Agency (2004) A manual for developing biogas systems at commercial farms in the United States: AgSTAR Handbook (EPA-430-B-97-015). Chapter 1 Overview of biogas technology. Retrieved from http://www2.epa.gov/sites/production/files/2014-12/documents/agstar-handbook.pdf
  4. 4.
    Holm-Nielsen JB, Ai Seadi T, Oleskowicz-Popielc P (2009) The future of anaerobic digestion and biogas utilization. Bioresour Technol 100(22):5478–5484CrossRefPubMedGoogle Scholar
  5. 5.
    Oswald JW, Gotaas HB, Golueke CG, Kellen WR (1957) Algae in waste treatment. Sewage Ind Waste 29(4):437–457Google Scholar
  6. 6.
    WEF (1990) Natural systems for wastewater treatment: manual of practice FD-16. Water Environment Federation, AlexandriaGoogle Scholar
  7. 7.
    Wang M, Yang H, Ergas S, van der Steen P (2015) A novel shortcut nitrogen removal process using an algal-bacterial consortium in a photo-sequencing batch reactor (PSBR). Water Res 87:38–48CrossRefPubMedGoogle Scholar
  8. 8.
    Mulbry W, Kondrad S, Buyer J (2008) Treatment of dairy and swine manure effluents using freshwater algae: fatty acid content and composition of algal biomass at different manure loading rates. J Appl Phycol 20(6):1079–1085CrossRefGoogle Scholar
  9. 9.
    Hu B, Min M, Zhou W, Du Z, Mohr M, Chen P, Zhu J, Cheng Y, Liu Y, Ruan R (2012) Enhanced mixotrophic growth of microalga Chlorella sp. on pretreated swine manure for simultaneous biofuel feedstock production and nutrient removal. Bioresour Technol 126:71–79CrossRefPubMedGoogle Scholar
  10. 10.
    Halfhide T, Dalrymple O, Wilkie A, Trimmer J, Gillie B, Udom I, Zhang Q, Ergas S (2014) Growth of an indigenous algal consortium on anaerobically digested municipal sludge centrate: photobioreactor performance and modeling. BioEnerg Res 8(1):249–258Google Scholar
  11. 11.
    Wang L, Li Y, Chen P, Min M, Chen Y, Zhu J, Ruan RR (2010) Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp. Bioresour Technol 101(8):2623–2628CrossRefPubMedGoogle Scholar
  12. 12.
    Yuan X, Wang M, Park C, Sahu AK, Ergas SJ (2012) Microalgae growth using high-strength wastewater followed by anaerobic co-digestion. Water Environ Res 84(5):396–404CrossRefPubMedGoogle Scholar
  13. 13.
    Udom I, Zaribaf BH, Halfhide T, Gillie B, Dalrymple O, Zhang Q, Ergas SJ (2013) Harvesting microalgae grown on wastewater. Bioresour Technol 139:101–106CrossRefPubMedGoogle Scholar
  14. 14.
    Astals S, Musenze RS, Bai X, Tannock S, Tait S, Pratt S, Jensen PD (2015) Anaerobic co-digestion of pig manure and algae: impact of intracellular algal products recovery on co-digestion performance. Bioresour Technol 181:97–104CrossRefPubMedGoogle Scholar
  15. 15.
    González-Fernández C, Molinuevo-Salces B, García-González MC (2011) Evaluation of anaerobic codigestion of microalgal biomass and swine manure via response surface methodology. Appl Energy 88(10):3448–3453CrossRefGoogle Scholar
  16. 16.
    APHA (American Public Health Association) (2012) Standard methods for the examination of water and wastewater 20th ed. American Public Health Association/American Water Works Association/Water Environment Federation, Washington DCGoogle Scholar
  17. 17.
    Bligh EG, Dyer WJ (1959) A rapid method pf tpta; lipid extraction and purification. Can J Biochem Physiol 37(8):911–917CrossRefPubMedGoogle Scholar
  18. 18.
    Mandal S, Mallick N (2009) Microalga Scenedesmus obliquus as a potential source for biodiesel production. Appl Microbiol Biotechnol 84(2):281–291CrossRefPubMedGoogle Scholar
  19. 19.
    Wang M, Park C (2015) Investigation of anaerobic digestion of Chlorella sp. and Micractinium sp. grown in high-nitrogen wastewater and their co-digestion with waste activated sludge. Biomass Bioenergy 80:30–37CrossRefGoogle Scholar
  20. 20.
    U.S. Environmental Protection Agency (2004) Managing manure nutrients at concentrated animal feeding operations, EPA-821-B-04-006, Chapter 5 Voluntary Performance Standards for CAFOs., pp 5-13–5-18Google Scholar
  21. 21.
    Hamilton DW, Luce WG, Heald AD (1997) Production and characteristics of swine manure. Oklahoma Cooperative Extension Service No. F-1735, StillwaterGoogle Scholar
  22. 22.
    Murphy CF, Allen DT (2011) Energy-water nexus for mass cultivation of algae. Environ Sci Technol 45(13):5861–5868CrossRefPubMedGoogle Scholar
  23. 23.
    Zupancic GD, Ros M (2003) Heat and energy requirements in thermophilic anaerobic sludge digestion. Renew Energy 28:2255–2267CrossRefGoogle Scholar
  24. 24.
    Tchobanoglous G, Burton FL, Stensel HD (2003) Treatment, reuse, and disposal of solids and biosolids. In: Wastewater engineering: treatment and reuse, 4th edn. McGraw-Hill, BostonGoogle Scholar
  25. 25.
    Collet P, Hélias A, Lardon L, Ras M, Goy RA, Steyer JP (2011) Life-cycle assessment of microalgae culture coupled to biogas production. Bioresour Technol 102(1):207–214CrossRefPubMedGoogle Scholar
  26. 26.
    Frost P, Gilkinson S (2010) Interim technical report: first 18 month performance summary for anaerobic digestion of dairy cow slurry at AFBI Hillsborough., Agri-Food and Biosciences Institute, Retrieved May 28, 2014, from http://www.afbini.gov.uk/afbi_ad_18_months-web.pdf Google Scholar
  27. 27.
    Pronto J, Gooch C (2012) Anaerobic digestion at Patterson Farms, Inc.: case study. Department of Biological and Environmental Engineering, Cornell University. Retrieved May 28, 2014, from http://www.manuremanagement.cornell.edu/Pages/General_Docs/Case_Studies/Patterson_case_study_revision_3.pdfGoogle Scholar
  28. 28.
    Crittenden JC, Trussell RR, Hand DW, Howe KJ, Tchobanoglous G (2012) Air stripping and aeration. In: MWH’s water treatment: principles and design. Wiley, Hoboken, NJ, pp. 1163-1244Google Scholar
  29. 29.
  30. 30.
    Reitan KI, Rainuzzo JR, Olsen Y (1994) Effect of nutrient limitation on fatty acid and lipid content of marine microalgae. J Phycol 30(6):972–979CrossRefGoogle Scholar
  31. 31.
    Sialve B, Bernet N, Bernard O (2009) Anaerobic digestion of microalgae as a necessary step to make microalgal biodiesel sustainable. Biotechnol Adv 27(4):409–416CrossRefPubMedGoogle Scholar
  32. 32.
    Dębowski M, Zieliński M, Grala A, Dudek M (2013) Algae biomass as an alternative substrate in biogas production technologies—review. Renew Sust Energ Rev 27:596–604CrossRefGoogle Scholar
  33. 33.
    Wang M, Sahu AK, Rusten B, Park C (2013) Anaerobic co-digestion of microalgae Chlorella sp. and waste activated sludge. Bioresour Technol 142:585–590CrossRefPubMedGoogle Scholar
  34. 34.
    Sakar S, Yetilmezsoy K, Kocak E (2009) Anaerobic digestion technology in poultry and livestock waste treatment—a literature review. Waste Manag Res 27(1):3–18CrossRefPubMedGoogle Scholar
  35. 35.
    Pittman JK, Dean AP, Osundeko O (2011) The potential of sustainable algal biofuel production using wastewater resources. Bioresour Technol 102(1):17–25CrossRefPubMedGoogle Scholar
  36. 36.
    Nielsen HB, Angelidaki I (2008) Strategies for optimizing recovery of the biogas process following ammonia inhibition. Bioresour Technol 99(17):7995–8001CrossRefPubMedGoogle Scholar
  37. 37.
    Lansing S, Martin JF, Botero RB, Nogueira da Silva T, Dias da Silva E (2010) Wastewater transformations and fertilizer value when co-digesting differing ratios of swine manure and used cooking grease in low-cost digesters. Biomass Bioenergy 34(12):1711–1720CrossRefGoogle Scholar
  38. 38.
    Manser ND, Mihelcic JR, Ergas SJ (2015) Semi-continuous mesophilic anaerobic digester performance under variations in solids retention time and feeding frequency. Bioresour Technol 190:359–366CrossRefPubMedGoogle Scholar
  39. 39.
    Smith SR, Lang NL, Cheung KHM, Spanoudaki K (2005) Factors controlling pathogen destruction during anaerobic digestion of biowastes. Waste Manag 25(4):417–425CrossRefPubMedGoogle Scholar
  40. 40.
    Kim JK, Oh BR, Chun YN, Kim SW (2006) Effects of temperature and hydraulic retention time on anaerobic digestion of food waste. J Biosci Bioeng 102(4):328–332CrossRefPubMedGoogle Scholar
  41. 41.
    Sahlstrom L (2003) A review of survival of pathogenic bacteria in organic waste used in biogas plants. Bioresour Technol 87(2):161–166CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Meng Wang
    • 1
  • Eunyoung Lee
    • 1
  • Qiong Zhang
    • 1
  • Sarina J. Ergas
    • 1
    Email author
  1. 1.Department of Civil & Environmental EngineeringUniversity of South FloridaTampaUSA

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