BioEnergy Research

, Volume 9, Issue 1, pp 239–247 | Cite as

Reduction of Ethanol Yield from Switchgrass Infected with Rust Caused by Puccinia emaculata

  • Virginia R. Sykes
  • Fred L. Allen
  • Jonathan R. Mielenz
  • C. Neal StewartJr.
  • Mark T. Windham
  • Choo Y. Hamilton
  • Miguel RodriguezJr.
  • Kelsey L. Yee
Article

Abstract

Switchgrass (Panicum virgatum) is an important biofuel crop candidate thought to have low disease susceptibility. As switchgrass production becomes more prevalent, monoculture and production fields in close proximity to one another may increase the spread and severity of diseases such as switchgrass rust caused by the pathogen Puccinia emaculata. The objective of this research was to examine the impact of rust on ethanol yield in switchgrass. In 2010 and 2012, naturally infected leaves from field-grown ‘Alamo’ and ‘Kanlow’ in Knoxville, TN (2010, 2012) and Crossville, TN (2012) were visually categorized as exhibiting low, medium, or high disease based on the degree of chlorosis and sporulation. P. emaculata was isolated from each disease range to confirm infection. Samples from 2010 were acid/heat pretreated and subjected to two runs of simultaneous saccharification and fermentation (SSF) with Saccharomyces cerevisiae D5A to measure ethanol yield. Near-infrared spectroscopy (NIRS) was used to estimate ethanol yield for 2012 samples. SSF and NIRS data were analyzed separately using ANOVA. Disease level effects were significant within both models (P < 0.05) and both models explained a large amount of variation in ETOH (SSF: R2 = 0.99, NIRS: R2 = 0.99). In the SSF dataset, ethanol was reduced by 35 % in samples exhibiting medium disease symptoms and by 55 % in samples exhibiting high disease symptoms. In the NIRS dataset, estimated ethanol was reduced by 10 % in samples exhibiting medium disease symptoms and by 21 % in samples exhibiting high disease symptoms. Results indicate that switchgrass rust will likely have a negative impact on ethanol yield in switchgrass grown as a biofuel crop.

Keywords

NIRS Rust Puccinia emaculata Switchgrass Ethanol SSF Panicum virgatum 

References

  1. 1.
    McLaughlin SB, Kszos LA (2005) Development of switchgrass (Panicum virgatum) as a bioenergy feedstock in the United States. Biomass Bioenergy 28(6):515–535CrossRefGoogle Scholar
  2. 2.
    Gravert CE, Munkvold GP (2002) Fungi and diseases associated with cultivated switchgrass in Iowa. J Iowa Acad Sci 109(1, 2):30–34Google Scholar
  3. 3.
    Zale J, Freshour L, Agarwal S, Sorochan J, Ownley BH, Gwinn KD, Castlebury LA (2008) First report of rust on switchgrass (Panicum virgatum) caused by Puccinia emaculata in Tennessee. Plant Dis 92(12):1710CrossRefGoogle Scholar
  4. 4.
    Cornelius DR, Johnston CO (1941) Differences in plant type and reaction to rust among several collections of Panicum virgatum. Agron J 33(2):115–124CrossRefGoogle Scholar
  5. 5.
    Hopkins AA, Vogel KP, Moore KJ, Johnson KD, Carlson IT (1995) Genotypic variability and genotype × environment interactions among switchgrass accessions from the midwestern USA. Crop Sci 35(2):565–571CrossRefGoogle Scholar
  6. 6.
    Cummins GB (1971) The rust fungi of cereals, grasses, and bamboos. Springer, New YorkCrossRefGoogle Scholar
  7. 7.
    Farr DE, Bills GF, Chamuris GP, Rossman AY (1995) Fungi on plants and plant products in the United States. APS, St PaulGoogle Scholar
  8. 8.
    Tiffany LH, Knaphus G (1985) The rust fungi (Uredinales) of the Loess Hills region of Iowa. J Iowa Acad Sci 92(5):186–188Google Scholar
  9. 9.
    Gilman JC, Archer WA (1929) The fungi of Iowa parasitic on plants. Iowa State Coll J Sci 3:299–507Google Scholar
  10. 10.
    Hagan AK, Akridge JR (2013) Efficacy of fungicides for the control of rust on switchgrass. In: Dumenyo K (ed) SNA Res. Conf. Pathology and Nematology, pp 201–204Google Scholar
  11. 11.
    Li Y, Windham M, Trigiano R, Windham A, Ownley B, Gwinn K, Zale J, Spiers J (2009) Cultivar-specific interactions between switchgrass and Puccinia emaculata. Phytopathology 99(6):S72Google Scholar
  12. 12.
    Garland CD (2008) Growing and harvesting switchgrass for ethanol production in Tennessee. UT Biofuels Initiative - SP701-A-5M-5/08, vol SP701-A-5M-5/08. UT Extension; Knoxville, TNGoogle Scholar
  13. 13.
    Moore KJ, Moser LE, Vogel KP, Waller SS, Johnson BE, Pedersen JF (1991) Describing and quantifying growth stages of perennial forage grasses. Agron J 83(6):1073–1077CrossRefGoogle Scholar
  14. 14.
    Dowe N, McMillan J (2001) SSF experimental protocols—lignocellulosic biomass hydrolysis and fermentation. Laboratory Analytical Procedure, vol NREL/TP-510-42630. National Renewable Energy LaboratoryGoogle Scholar
  15. 15.
    Fu C, Mielenz JR, Xiao X, Ge Y, Hamilton CY, Rodriguez M, Chen F, Foston M, Ragauskas A, Bouton J, Dixon RA, Wang Z (2011) Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass. PNAS 108(9):3803–3808PubMedCentralCrossRefPubMedGoogle Scholar
  16. 16.
    Murray I, Cowe I (2004) Sample preparation. In: Roberts CA, Workman J, Reeves JB (eds) Near-infrared spectroscopy in agriculture, vol 44. American Society of Agronomy, Inc., Crop Science Society of America, Inc., Soil Science Society of America, Inc., Madison, WI, pp 75–112Google Scholar
  17. 17.
    Vogel KP, Dien BS, Jung HG, Casler MD, Masterson SD, Mitchell RB (2011) Quantifying actual and theoretical ethanol yields for switchgrass strains using NIRS analyses. Bioenergy Res 4:96–110CrossRefGoogle Scholar
  18. 18.
    Mixed hay: NIRS Forage and Feed Testing Consortium, June 2007 mixed hay calibration, file name: mh50-3. Parameters used: DM, CP, ADF, dNDF48, NDF, Ca, P, K, Mg, ash, fat, lignin, RUPGoogle Scholar
  19. 19.
    Lorenz AJ, Anex RP, Isci A, Coors JG, de Leon N, Weimer PJ (2009) Forage quality and composition measurements as predictors of ethanol yield from maize (Zea mays L.) stover. Biotechnol Biofuels 2(1):5PubMedCentralCrossRefPubMedGoogle Scholar
  20. 20.
    SAS 9.3 TS Level 1M2. SAS Institute, CaryGoogle Scholar
  21. 21.
    Arthur JC (1934) Manual of rusts in United States and Canada. Science Press, LancasterGoogle Scholar
  22. 22.
    Jung HG, Vogel KP (1992) Lignification of switchgrass (Panicum virgatum) and big bluestem (Andropogon gerardii) plant parts during maturation and its effect on fibre degradability. J Sci Food Agric 59:169–176CrossRefGoogle Scholar
  23. 23.
    Wilson JP, Gates RN, Hanna WW (1991) Effect of rust on yield and digestibility of pearl millet forage. Phytopathology 81(2):233–236CrossRefGoogle Scholar
  24. 24.
    Queiroz OCM, Kim SC, Adesogan AT (2012) Effect of treatment with a mixture of bacteria and fibrolytic enzymes on the quality and safety of corn silage infested with different levels of rust. J Dairy Sci 95(9):5285–5291CrossRefPubMedGoogle Scholar
  25. 25.
    Johnson JC, Gates RN, Newton GL, Wilson JP, Chandler LD, Utley PR (1997) Yield, composition, and in vitro digestibility of temperate and tropical corn hybrids grown as silage crops planted in summer. J Dairy Sci 80(3):550–557CrossRefPubMedGoogle Scholar
  26. 26.
    Manners M (1982) Pathways of glucose assimilation in Puccinia graminis. J Gen Microbiol 128(11):2621–2630Google Scholar
  27. 27.
    Bartnicki-Garcia S (1968) Cell wall chemistry, morphogenesis, and taxonomy of fungi. Annu Rev Microbiol 22:87–108CrossRefPubMedGoogle Scholar
  28. 28.
    Silva MC, Nicole M, Rijo L, Geiger JP, Rodrigues CJ (1999) Cytochemical aspects of the plant-rust fungus interface during the compatible interaction Coffea arabica (cv. Caturra)–Hemileia vastarix (race III). Int J Plant Sci 160(1):79–91CrossRefGoogle Scholar
  29. 29.
    Hammerschmidt R (1984) Rapid deposition of lignin in potato tuber tissue as a response to non-pathogenic fungi on potato. Physiol Plant Pathol 24(1):33–42CrossRefGoogle Scholar
  30. 30.
    Zhang SH, Yang Q, Ma RC (2007) Erwinia carotovora spp. carotovora infection induced “defense lignin” accumulation and lignin biosynthetic gene expression in Chinese cabbage (Brassica rapa L. spp pekinensis). J Integr Plant Biol 49(7):993–1002CrossRefGoogle Scholar
  31. 31.
    Liu XY, Jin JY, He P, Gao W, Li WJ (2007) Effect of potassium chloride on lignin metabolism and its relation to resistance of corn to stalk rot. Sci Agric Sin 40:2780–2787Google Scholar
  32. 32.
    Karkonen A, Koutaniemi S (2010) Lignin biosynthesis studies in plant tissue cultures. J Integr Plant Biol 52(2):176–185CrossRefPubMedGoogle Scholar
  33. 33.
    Ride JP (1978) The role of cell wall alterations in resistance to fungi. Ann Appl Biol 89:302–306Google Scholar
  34. 34.
    Xu H, Heath MC (1998) Role of calcium in signal transduction during the hypersensitive response caused by basidiospore-derived infection of the cowpea rust fungus. Plant Cell 10(4):585–597PubMedCentralCrossRefPubMedGoogle Scholar
  35. 35.
    Liu G, Hou CY, Wang DM (2010) Calcium influx is required for the initiation of the hypersensitive response of Triticum aestivum to Puccinia recondite f.sp. tritici. Physiol Mol Plant Pathol 74(3–4):267–273CrossRefGoogle Scholar
  36. 36.
    Chotineeranat S, Wansuksri R, Piyachomkwan K, Chatakanonda P, Weerathaworn P, Sriroth K (2010) Effect of calcium ions on ethanol production from molasses by Saccharomyces cerevisiae. Sugar Technol 12(2):120–124CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Virginia R. Sykes
    • 1
  • Fred L. Allen
    • 1
  • Jonathan R. Mielenz
    • 2
    • 5
  • C. Neal StewartJr.
    • 1
    • 2
  • Mark T. Windham
    • 3
  • Choo Y. Hamilton
    • 2
    • 6
  • Miguel RodriguezJr.
    • 2
    • 4
  • Kelsey L. Yee
    • 2
    • 7
  1. 1.Department of Plant SciencesUniversity of TennesseeKnoxvilleUSA
  2. 2.Bioenergy Science CenterOak Ridge National LaboratoryOak RidgeUSA
  3. 3.Department of Entomology and Plant PathologyUniversity of TennesseeKnoxvilleUSA
  4. 4.Bioconversion Science & Technology BioSciences DivisionOak Ridge National LaboratoryOak RidgeUSA
  5. 5.White Cliff BiosystemsRockwoodUSA
  6. 6.Center for Renewable CarbonKnoxvilleUSA
  7. 7.Genomatica Inc.San DiegoUSA

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