Abstract
Chemical 2,3-butanediol is an important platform compound possessing diverse industrial applications. So far, it is mainly produced by using petrochemical feedstock which is associated with high cost and adverse environmental impacts. Hence, finding alternative routes (e.g., via fermentation using renewable carbon sources) to produce 2,3-butanediol are urgently needed. In this study, we report a wild-type Klebsiella sp. strain XRM21, which is capable of producing 2,3-butanediol from a wide variety of carbon sources including glucose, sucrose, xylose, and glycerol. Among them, fermentation of sucrose leads to the highest production of 2,3-butanediol. To maximize the production of 2,3-butanediol, fermentation conditions were first optimized for strain XMR21 by using response surface methodology (RSM) in batch reactors. Subsequently, a fed-batch fermentation strategy was designed based on the optimized parameters, where 91.2 g/L of 2,3-butanediol could be produced from substrate sucrose dosing in 100 g/L for three times. Moreover, random mutagenesis of stain XMR21 resulted in a highly productive mutant strain, capable of producing 119.4 and 22.5 g/L of 2,3-butanediol and ethanol under optimized fed-batch fermentation process within 65 h with a total productivity of 2.18 g/L/h, which is comparable to the reported highest 2,3-butanediol concentration produced by previous strains. This study provides a potential strategy to produce industrially important 2,3-butanediol from low-cost sucrose.
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This research is supported by the National Research Foundation, Prime Minister’s Office, Singapore, under the Competitive Research Programme with a Project No. NRF-CRP5-2009-05.
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Highlights
• A butanediol-producing bacterium Klebsiella sp. strain XMR21 was isolated.
• This strain showed diverse carbon utilization spectrum and could produce 2,3-butanediol and ethanol as its end product.
• Fermentation conditions were optimized using response surface methodology.
• Under optimized fed-batch fermentation, 91.2 g/L 2,3-butanediol could be produced.
• Strain improvement could further improve the production of 2,3-butanediol and ethanol to 119.4 and 22.5 g/L, which is comparable to the reported highest 2,3-butanediol concentration produced by previous strains.
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Xin, F., Basu, A., Weng, M.C. et al. Production of 2,3-Butanediol from Sucrose by a Klebsiella Species. Bioenerg. Res. 9, 15–22 (2016). https://doi.org/10.1007/s12155-015-9653-7
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DOI: https://doi.org/10.1007/s12155-015-9653-7