A Multi-Level Analysis Approach to Measuring Variations in Biomass Recalcitrance of Douglas Fir Tree Samples
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Biomass recalcitrance is a major bottleneck in the development of an economically viable process to convert woody biomass into fuels and other valuable chemicals. Selective breeding of trees with low recalcitrance toward biofuel conversion could help significantly reduce the cost of biofuel production, but such efforts would require a greater understanding of the nature of variations in the biomass recalcitrance of softwood species. The complexity of biomass recalcitrance, however, hinders research into determining the viability of breeding programs aimed to improve the recalcitrance of softwoods. In this study, a method was developed to determine biomass recalcitrance at three levels: chemical composition, pretreatment yield, and sugar release from the enzymatic hydrolysis. This method is designed to investigate the biomass recalcitrance variations among different families of Douglas-fir, which is the most abundant and promising softwood species for biofuel production in the Pacific Northwest region. Wood samples from 150 plantation-grown trees were collected and analyzed to test the method and the applicability of the parameters screened. The relationships between these levels are discussed to determine the best method for screening large D. fir populations. A parameter, a biomass recalcitrance factor, was introduced to quantify the level of biomass recalcitrance toward sugar production from different D. fir trees.
KeywordsBiomass recalcitrance Douglas fir Biomass composition Multi-level biomass analysis
Funding for this study is provided through the Northwest Advanced Renewables Alliance Project, supported by an Agriculture and Food Research Initiative Competitive Grant No. 2011-68005-30416 from the USDA National Institute of Food and Agriculture.
- 3.Brent, D., and S. Rabotyagov (2013) Land use change from biofuels derived from forest residue. Economics Research International. 2013Google Scholar
- 5.Howe GT, Jayawickrama K, Cherry M, Johnson GR, Wheeler NC (2006) Breeding Douglas-fir. In: Janick J (ed) Plant Breeding Reviews, Vol 27. Wiley-Blackwell, Commerce Place, 350 Main Street, Malden 02148, Ma USA, City, pp 245–353Google Scholar
- 15.Nystedt, B., N. R. Street, A. Wetterbom, A. Zuccolo, Y.-C. Lin, D. G. Scofield, F. Vezzi, N. Delhomme, S. Giacomello, A. Alexeyenko, R. Vicedomini, K. Sahlin, E. Sherwood, M. Elfstrand, L. Gramzow, K. Holmberg, J. Hallman, O. Keech, L. Klasson, M. Koriabine, M. Kucukoglu, M. Kaller, J. Luthman, F. Lysholm, T. Niittyla, A. Olson, N. Rilakovic, C. Ritland, J. A. Rossello, J. Sena, T. Svensson, C. Talavera-Lopez, G. Theiszen, H. Tuominen, K. Vanneste, Z.-Q. Wu, B. Zhang, P. Zerbe, L. Arvestad, R. Bhalerao, J. Bohlmann, J. Bousquet, R. Garcia Gil, T. R. Hvidsten, P. de Jong, J. MacKay, M. Morgante, K. Ritland, B. Sundberg, S. Lee Thompson, Y. Van de Peer, B. Andersson, O. Nilsson, P. K. Ingvarsson, J. Lundeberg, and S. Jansson (2013) The Norway spruce genome sequence and conifer genome evolution. Nature. advance online publicationGoogle Scholar
- 21.Sluiter, A., B. Hames, R. Ruiz, C. Scarlata, J. Sluiter, D. Templeton, and D. Crocker (2008) Determination of structural carbohydrates and lignin in biomass. In: Editor (ed.)^(eds.)|. Book Title|. Publisher|, City|Google Scholar
- 22.Wise LE, Murphy M, D'Addieco AA (1946) Chlorite holocellulose, its fractionation and bearing on summative wood analysis and on studies on the hemicelluloses. Paper Tr J 122:35–43Google Scholar
- 23.Timell TE (1959) Isolation of holocellulose from Jack pine Pinus banksiana. Pulp Paper Mag Can 60:T26–T28Google Scholar
- 27.Back EL (2000) The locations and morphology of resin components in the wood. In: Back EL, Allen LH (eds) Pitch Control, Wood Resin and Deresination. TAPPI Press, USAGoogle Scholar
- 29.Nakagame, S., R. P. Chandra, and J. N. Saddler (2011) The influence of lignin on the enzymatic hydrolysis of pretreated biomass substrates. pp. 145-167In: J. J. Y. Zhu, X. Zhang, and X. J. Pan (eds.). Sustainable Production of Fuels, Chemicals, and Fibers from Forest Biomass. Amer Chemical Soc, 1155 Sixteenth St Nw, Washington, Dc 20036 USA, CityGoogle Scholar