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Enhancing Lipid Production from Crude Glycerol by Newly Isolated Oleaginous Yeasts: Strain Selection, Process Optimization, and Fed-Batch Strategy

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Abstract

High lipid-accumulating yeast Trichosporonoides spathulata was newly isolated using crude glycerol as a sole carbon source. After process optimization in a 5-L bioreactor equipped with pH control and aeration system, T. spathulata produced biomass of 11.3 g/L and lipid of 5.01 g/L with a lipid content of 44.3 % using 10 % (w/v) of crude glycerol supplemented only with 0.5 % (w/v) of ammonium sulfate. A one-stage fed-batch feeding with crude glycerol and ammonium sulfate enhanced biomass and lipid production up to 17.3 and 7.25 g/L, respectively, with a lipid content of 41.9 %, while a two-stage fed-batch feeding with only crude glycerol in the second stage led to a lower biomass of 13.8 g/L but a higher lipid production of 7.78 g/L and a higher lipid content of 56.4 %. The fatty acid composition of produced lipid that is similar to plant oil indicates the high potential use of T. spathulata lipid as biodiesel feedstocks.

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Acknowledgments

This research was financially supported by the Graduate School of Prince of Songkla University. The first author thanks Palm Oil Products and Technology Research Center (POPTEC) for providing the scholarship. This work was also supported by the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission. Thanks also to Dr. Brian Hodgson for his assistance with the English.

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Authors and Affiliations

  1. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand

    Suleeporn Kitcha & Benjamas Cheirsilp

  2. Palm Oil Products and Technology Research Center (POPTEC), Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand

    Benjamas Cheirsilp

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  1. Suleeporn Kitcha
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  2. Benjamas Cheirsilp
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Correspondence to Benjamas Cheirsilp.

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Kitcha, S., Cheirsilp, B. Enhancing Lipid Production from Crude Glycerol by Newly Isolated Oleaginous Yeasts: Strain Selection, Process Optimization, and Fed-Batch Strategy. Bioenerg. Res. 6, 300–310 (2013). https://doi.org/10.1007/s12155-012-9257-4

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