Abstract
Objective
The main aim of this investigation is the clinical application of ultrasound irradiation technique as an alternative method to reconstitute sestamibi kits in comparison of water boiling bath method.
Methods
The 740–3700 MBq (20–100 mCi) 99mTc-MIBI (sestamibi) complex samples were prepared due to ultrasound irradiation technique or boiled water bath method as a standard method. Twenty patients (8 men and 12 women; age range 30–72, median 52.45 years) have been referred to Golestan hospital for myocardial perfusion imaging (MPI). The subjects have been divided randomly into group A (3 men, 7 women, age range 36–67, median 51.7 years) and group B (5 men, 5 women, age range 30–72, median 50.3 years), respectively. The 99mTc-MIBI radiopharmaceuticals have been prepared by Ultrasound irradiation technique administrated to group A and 99mTc-MIBI complex samples due to the boiled water bath technique administrated to the other group. For all patients, the 2-day stress/rest MPI protocol was performed.
Results
The radio-HPLC and TLC studies have indicated that the 99mTc-MIBI complex samples with good yields could be prepared successfully due to new developed technique. The scintigraphy imaging studies have demonstrated that the 99mTc-sestamibi prepared due to the above-mentioned modalities shows very identical biodistribution in the heart, thyroid, lung, liver, gallbladder, kidneys, stomach, large intestine and bladder of the subjects. Any unexpected accumulation of radiotracer samples have not been observed in our approach.
Conclusions
The ultrasound irradiation technique is convenient and sufficient method to prepare 99mTc-sestamibi. It can be recommended as an alternative method to reconstitute sestamibi kits particularly in emergency situations to reduce potentially medical risk by avoiding any delay in acute therapy for myocardial infarction.
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Acknowledgments
This approach is part of pharm-D thesis of Behzad Norouzi and special thanks go to Ahvaz Jundishapur University of Medical Sciences for the financial support and also to Radioisotope Division of Atomic Energy Organization of Iran for providing the freeze-dried sestamibi kits and 99Mo/99mTc generator.
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Doroudi, A., Erfani, M., Norouzi, B. et al. Clinical application of ultrasound for preparation of 99mTc-sestamibi complex. Ann Nucl Med 29, 295–301 (2015). https://doi.org/10.1007/s12149-014-0941-7
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DOI: https://doi.org/10.1007/s12149-014-0941-7