Abstract
Purpose
The purpose of our study was to evaluate the degree of radiotoxicity to lymphocytes in thyroid cancer after iodine-131(I-131) therapy using γ-H2AX foci immunodetection.
Methods
This study focused on 15 patients who underwent I-131 therapy for differentiated thyroid cancer after surgery. All patients received 3.7 GBq of I-131. Venous blood samples were collected from each patient before therapy and 4 days thereafter. Lymphocytes were isolated from the blood samples and subjected to γ-H2AX immunofluorescence staining.
Results
The number (mean ± SD) of foci per lymphocyte nucleus was 0.41 ± 0.51 before and 6.19 ± 1.80 after radioiodine therapy, and this difference was statistically significant (P = 0.001 < 0.05). Absorbed doses estimated for the 15 patients were 0.77 ± 0.31 Gy applying standard line in vitro external radiation doses.
Conclusion
γ-H2AX foci immunodetection in lymphocytes may detect radiation-induced DNA damage associated with I-131 therapy for thyroid cancer, and may facilitate estimation of the radiation doses absorbed with this therapy.
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Acknowledgments
This study was supported in part by a Grant-in-Aid for scientific research from the Japan Society for the promotion of Science, Grant for Collaborative Research from Kanazawa Medical University (C2009-5,C2010-3), Grant for Project Research from High-Tech Research Center of Kanazawa Medical University (H2010-10, H2011-10), Assist KAKEN from Kanazawa Medical University and Hokkoku foundation for cancer research.
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Doai, M., Watanabe, N., Takahashi, T. et al. Sensitive immunodetection of radiotoxicity after iodine-131 therapy for thyroid cancer using γ-H2AX foci of DNA damage in lymphocytes. Ann Nucl Med 27, 233–238 (2013). https://doi.org/10.1007/s12149-012-0678-0
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DOI: https://doi.org/10.1007/s12149-012-0678-0