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Comparison of animal studies between interstitial magnetic resonance lymphography and radiocolloid SPECT/CT lymphoscintigraphy in the head and neck region

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Abstract

Objective

To comparatively assess two techniques, radiocolloid SPECT/CT lymphoscintigraphy and interstitial MR lymphography using SPIO and gadoxetate disodium, in animal models.

Materials and methods

We used twenty one 8-week-old male nude mice of strain BALB/c Slc-nu/nu, weighing 23–27 g. The 4.7-T MRI equipment was used to detect the SNs. T2*WI of gradient-echo sequences was acquired sequentially up to 24 h after administering SPIO, ferucarbotran. T1WI was acquired sequentially up to 80 min after administering gadoxetate disodium. 99mTc-phytate SPECT/CT lymphoscintigraphy was taken at 30 min after the injection to detect the SNs using animal-dedicated whole-body SPECT/CT hybrid scanner. The injection was submucosally performed in the right tongue margin of each mouse. Reading performances concerning SN visualization and its quality on interstitial MR lymphogram and SPECT/CT lymphoscintigram were performed by 3 radiologists.

Results

The SN intensities were 0.43 for the right, 0.61 for the left at 30 min after ferucarbotran injection, with gradual decrease in intensity, and 1.43 for the right, 1.33 for the left at 10 min after gadoxetate disodium injection with a fast decrease in intensity. The base value of 1.0 was at pre-examination. The mean numbers of lymph nodes visualized were 4.00 nodes for on SPECT/CT lymphoscintigram and 2.0 for interstitial MR lymphogram. There was a statistically significant difference in the mean scores between SPECT/CT lymphoscintigraphy and interstitial MR lymphography (two factor mixed design with repeated measures on one factor: p < 0.0002).

Conclusions

In our comparative study using mice, the results of radiocolloid SPECT/CT lymphoscintigraphy were superior to those of interstitial MR lymphography, while both SPIO and gadoxetate disodium have a potential of being employed for sentinel node navigation surgery by interstitial MR lymphography in the head and neck region.

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Acknowledgments

This study was funded by a grant from the 2009 Health, Welfare, Labor, Science Ministry Research Subsidy (2009-cancer clinical general, 016). The authors are indebted to the BioView Co. for the invaluable technical assistance and skills.

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Authors and Affiliations

  1. Department of Radiotherapy, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo, Japan

    Naoto Kitamura

  2. Department of Radiology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan

    Shigeru Kosuda & Hiroshi Shinmoto

  3. Department of Otolaryngology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, Japan

    Koji Araki, Masayuki Tomifuji, Daisuke Mizokami & Akihiro Shiotani

  4. Functional Imaging Division, Research Center for Innovative Oncology, National Cancer Center Hospital East, 6-5-1 Kashiwanoha, Kashiwa, Chiba, Japan

    Hirofumi Fujii

  5. Department of Clinical Laboratory Science, Faculty of Health Science, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi, Japan

    Kiyoshi Ichihara

Authors
  1. Naoto Kitamura
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  2. Shigeru Kosuda
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  3. Koji Araki
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  4. Masayuki Tomifuji
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  5. Daisuke Mizokami
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  6. Akihiro Shiotani
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  7. Hiroshi Shinmoto
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  8. Hirofumi Fujii
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  9. Kiyoshi Ichihara
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Corresponding author

Correspondence to Shigeru Kosuda.

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Kitamura, N., Kosuda, S., Araki, K. et al. Comparison of animal studies between interstitial magnetic resonance lymphography and radiocolloid SPECT/CT lymphoscintigraphy in the head and neck region. Ann Nucl Med 26, 281–285 (2012). https://doi.org/10.1007/s12149-011-0565-0

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  • DOI: https://doi.org/10.1007/s12149-011-0565-0

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