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VEGF-A and its isoform VEGF121 mRNA expression measured by quantitative real-time RT-PCR: correlation with F-18 FDG uptake and aggressiveness of lung adenocarcinoma: preliminary study

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Abstract

Objective

To investigate the correlation of vascular endothelial growth factor (VEGF) A and its isoform VEGF 121 mRNA expression with F-18 fluorodeoxyglucose (FDG) uptake and aggressiveness in lung adenocarcinoma.

Methods

Twenty-three patients with lung adenocarcinoma underwent FDG PET before surgery. As semi-quantitative analysis for FDG uptake, partial volume corrected standardized uptake value (PVC-SUV) of the tumor was calculated. Total RNA from lung adenocarcinoma tissue was prepared from the frozen specimens. Using the real-time reverse transcription polymerase chain reaction method, we analyzed the mRNA level of VEGF-A and VEGF-A isoform VEGF121 mRNA level. 18S ribosomal RNA was used as an endogenous control.

Results

VEGF-A and VEGF121 mRNA levels had significantly positive correlation with PVC-SUV in lung adenocarcinoma (r = 0.477, p = 0.021, r = 0.539, p = 0.008, respectively), while they were not correlated with tumor size (≤3 or >3 cm). VEGF-A and VEGF121 mRNA levels of the low FDG uptake group were significantly lower than those of the high FDG uptake group (p = 0.005 and p = 0.004, respectively). FDG uptake (PCV-SUV) of aggressive lung adenocarcinoma was higher than that of non-aggressive lung adenocarcinoma (p = 0.01). VEGF-A and VEGF121 mRNA levels of aggressive lung adenocarcinoma were higher than those of non-aggressive lung adenocarcinoma (p = 0.0001 and p = 0.0001, respectively).

Conclusion

VEGF-A and VEGF121 mRNA levels may correlate with FDG uptake and aggressiveness in lung adenocarcinoma. These findings support the hypothesis that VEGF-A and VEGF121 may help in predicting the outcome in patients with lung adenocarcinoma.

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Acknowledgments

This work was supported by a Grant for Project Research from High-Tech Research Center of Kanazawa Medical University (H2010-12, H2009-12, H2008-12, H2007-12, H2008-11, H2008-10, H2007-10, S2005-6, S2006-2), by The Policy Based Medical Services Network from the National Hospital Organization, and by a Grant-in Aid (19590370) for scientific research from the Ministry of Education, Japan.

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Authors and Affiliations

  1. Department of Radiology, Kanazawa Medical University, Ishikawa, Japan

    JianFei Guo, Kotaro Higashi, Lina Zhang & Hisao Tonami

  2. Department of Radiology, First Affiliated Hospital, China Medical University, Shenyang, People’s Republic of China

    JianFei Guo, Lina Zhang & Ke Xu

  3. Department of Radiology, Asanogawa General Hospital, 83 Kosakamachi-Naka, Kanazawa, Ishikawa, 920-8621, Japan

    Kotaro Higashi & Hiroto Nishida

  4. Department of Pathology, Kanazawa Medical University, Uchinada, Japan

    Yoshimichi Ueda

  5. Medical Research Institute, Kanazawa Medical University, Ishikawa, Japan

    Yasuhito Ishigaki & Tsutomu Takegami

  6. Department of Thoracic Surgery, Kanazawa Medical University, Ishikawa, Japan

    Tsutomu Sakuma

  7. Department of Radiology, Public Central Hospital of Matto Ishikawa, Ishikawa, Japan

    Manabu Oguchi

  8. Department of Thoracic Surgery, Kanazawa Medical Center, Ishikawa, Japan

    Yasuhiko Ohta

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  1. JianFei Guo
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Correspondence to Kotaro Higashi.

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Guo, J., Higashi, K., Ueda, Y. et al. VEGF-A and its isoform VEGF121 mRNA expression measured by quantitative real-time RT-PCR: correlation with F-18 FDG uptake and aggressiveness of lung adenocarcinoma: preliminary study. Ann Nucl Med 25, 29–36 (2011). https://doi.org/10.1007/s12149-010-0427-1

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  • DOI: https://doi.org/10.1007/s12149-010-0427-1

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