Abstract
The generation and extraction of the charged particles (ions, clusters) from a fluid in a strong electric field at ambient conditions using a dynamic liquid flow splitter on the meniscus was examined. The existence of non-droplets regimes for obtaining charged particles for liquid flow rates up to 1000 μL/min, solute concentrations up to 1 M and acid content in solution up to 1% was displayed. Depending on the experimental conditions a conventional fluid electrospraying regime at ambient conditions in air as well as a dropless regime were demonstrated for obtaining charged particles current. The performance of the ion mobility spectrometer with a Bradbury-Nielsen gate at a dropless mode of charged particles generation was demonstrated for solutions of Human serum albumin (69,367 Da), Apoferritin (445,000 Da) and human epidermoid carcinoma A431 cells. The concentration of each substance in the solutions was ranging from 10−4 to 10−5 M. The concentration of cells in the suspension was 1 million/mL.
Similar content being viewed by others
References
Arseniev AN, Krasnov NV, Muradymov MZ (2014) Investigation of electrospray stability with dynamic liquid flow splitter. J Anal Chem 69(14):1320–1322
Coy SL, Krylov EV, Nazarov EG, Fornace AY Jr, Kidd RD (2013) Differential mobility spectrometry with nanospray ion sourse as a compact detector for small organics and inorganics. Int J Ion Mobil Spectrom 16(3):217–227
Garabedian A, Bolufer A, Leng F, Fernandez-Lima F (2018) Peptide sequence influence on the conformational dynamics and DNA binding of the intrinsically disordered AT-hook 3 peptide. Sci Rep 8(1):10783
Krasnov NV, Muradymov MZ, Samokish VA (2013) Electrospray ion sourse with a dynamic liquid flow splitter. Rapid Commun Mass Spectrom 27:904–908
Kurnin IV, Krasnov NV, Semenov SY, Smirnov VN (2014) Bradbery-Nilsen gate electrode potential switching modes opti-mizing the ion packet time width in an ion mobility spectrometer. Int J Ion Mobil Spectrom 17(2):79–85
Merkley ED, Baker ES, Crowell KL, Orton DJ, Taverner T, Ansong C, Ibrahim YM, Burnet MC, Cort JR, Anderson GA, Smith RD, Adkins JN (2013) Mixed-isotope labeling with LC-IMS-MS for characterization of protein-protein interactions by chemical cross-linking. J Am Soc Mass Spectrom 24(3):444–449
Acknowledgments
The work was mainly carried out at the Institute for Analytical Instrumentation of the Russian Academy of Sciences, Saint Petersburg, in the framework of the state task № 075-00780-19-00.
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Al-Tavil, E.A., Krasnov, N.V., Muradymov, M.Z. et al. Dropless ESI for IMS at ambient conditions. Int. J. Ion Mobil. Spec. 22, 85–91 (2019). https://doi.org/10.1007/s12127-019-00250-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12127-019-00250-2