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Rapid analysis of N-methylpyrrolidine in cefepime with thermal desorption ion mobility spectrometry

  • Original Research
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International Journal for Ion Mobility Spectrometry

Abstract

N-methyl-pyrrolidine (NMP) a potential impurity in the cephalosporin antibiotic cefepime is analysed using a rapid thermal desorption – ion mobility spectrometry (TD-IMS) method. The thermal desorption approach is shown to be capable of rapidly extracting NMP from the cefepime at 80 °C without causing thermal degradation of the cefepime. The ion mobility method has an analysis time of 1 min and demonstrates good linearity over a range from 0.3–3.0 μg ml−1 of NMP, with limits of detection and quantification of 0.056 and 0.1875 μg ml−1 respectively. The developed method was applied to the analysis of a cefepime sample and determined that NMP was present in a cefepime sample at a level of 0.0376 % with a percentage relative standard deviation (n = 6) of 3.2 %. This was compared with a LC-UV method which was in close agreement measuring NMP at 0.0384 % in the cefepime sample with a percentage RSD (n = 6) of 5.7 %. These results show that the TD-IMS method gives comparable data to the established LC methods and demonstrates the potential of TD-IMS for rapid measurement of volatile compounds in pharmaceutical matrices.

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Authors and Affiliations

  1. Centre for Analytical Science, Department of Chemistry, Loughborough University, Ashby Road, Loughborough, LE11 3TU, UK

    James C. Reynolds, Laura Giddings, Ime C. Usen, Haodong Cheng, Junmin Shan, Matthew A. Turner & Colin S. Creaser

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  1. James C. Reynolds
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  2. Laura Giddings
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  3. Ime C. Usen
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  4. Haodong Cheng
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  5. Junmin Shan
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  6. Matthew A. Turner
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  7. Colin S. Creaser
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Correspondence to James C. Reynolds.

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Reynolds, J.C., Giddings, L., Usen, I.C. et al. Rapid analysis of N-methylpyrrolidine in cefepime with thermal desorption ion mobility spectrometry. Int. J. Ion Mobil. Spec. 19, 209–217 (2016). https://doi.org/10.1007/s12127-016-0210-7

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  • DOI: https://doi.org/10.1007/s12127-016-0210-7

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