Abstract
Toxoplasmosis is a systematic protozoan disease caused by a tiny parasite Toxoplasma gondii. The infection can be dangerous for pregnant woman and people with weak immune systems. The secreted protein named TgPDCD5 (Programmed cell death protein 5 from Toxoplasma gondii) plays an important role in apoptosis-inducing effect on host cells. Here, we report the 1H, 13C, and 15N resonance assignments of TgPDCD5. This work provides the ground for further structural elucidate and biophysical investigation about protein function.
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References
Aleixo AL, Curi AL, Benchimol EI, Amendoeira MR (2016) Toxoplasmic retinochoroiditis: clinical characteristics and visual outcome in a prospective study. PLoS Negl Trop Dis 10:e0004685
Bannai H, Nishikawa Y, Matsuo T, Kawase O, Watanabe J, Sugimoto C, Xuan X (2008) Programmed cell death 5 from Toxoplasma gondii: a secreted molecule that exerts a pro-apoptotic effect on host cells. Mol Biochem Parasitol 159:112–120
Bannai H et al (2009) Overproduction of the pro-apoptotic molecule, programmed cell death 5, in Toxoplasma gondii leads to increased apoptosis of host macrophages. J Vet Med Sci 71:1183–1189
Brunet J et al (2008) Toxoplasma gondii exploits UHRF1 and induces host cell cycle arrest at G2 to enable its proliferation. Cell Microbiol 10:908–920
Chang S et al (2015) Toxoplasma gondii rhoptry protein ROP16 mediates partially SH-SY5Y cells apoptosis and cell cycle arrest by directing Ser15/37 phosphorylation of p53. Int J Biol Sci 11:1215–1225
Gavrilescu LC, Denkers EY (2001) IFN-gamma overproduction and high level apoptosis are associated with high but not low virulence Toxoplasma gondii infection. J Immunol 167:902–909
Guntert P (2004) Automated NMR structure calculation with CYANA. Methods Mol Biol 278:353–378
Maenz M, Schluter D, Liesenfeld O, Schares G, Gross U, Pleyer U (2014) Ocular toxoplasmosis past, present and new aspects of an old disease. Prog Retin Eye Res 39:77–106
Markley JL et al (1998) Recommendations for the presentation of NMR structures of proteins and nucleic acids. IUPAC-IUBMB-IUPAB inter-union task group on the standardization of data bases of protein and nucleic acid structures determined by NMR spectroscopy. J Biomol NMR 12:1–23
Martin S (2001) Congenital toxoplasmosis. Neonatal Netw 20:23–30
Morjaria S et al (2016) Toxoplasma encephalitis in atypical hosts at an academic cancer center. Open Forum Infect Dis 3:ofw070
Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44:213–223
Soheilian M et al (2011) Randomized trial of intravitreal clindamycin and dexamethasone versus pyrimethamine, sulfadiazine, and prednisolone in treatment of ocular toxoplasmosis. Ophthalmology 118:134–141
Acknowledgements
The NMR spectra were obtained at the High-field NMR Center, Academia Sinica (AS-CFII-108-112). This work is supported by National Taiwan University (NTU 108L7861 and NTU 109L7734) and the Ministry of Science and Technology, Taiwan (MOST 108-2113-M-002-011 and MOST 108-2628-B-002-013).
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Lin, MH., Yu, TA., Chang, CF. et al. NMR resonance assignments of the programmed cell death protein 5 (PDCD5) from Toxoplasma gondii. Biomol NMR Assign 14, 277–280 (2020). https://doi.org/10.1007/s12104-020-09961-8
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DOI: https://doi.org/10.1007/s12104-020-09961-8