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1H, 13C and 15N resonance assignments of TFIIS LW domain from Homo sapiens

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Abstract

LW domain is the N-terminal domain I of transcription elongation factor TFIIS, which is a component of RNA polymerase II (Pol II) preinitiation complexes (PICs). Here, we report the resonance assignments of TFIIS LW domain from Homo sapiens for further understanding of the relationship between its structure and function.

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References

  • Booth V, Koth CM, Edwards AM, Arrowsmith CH (2000) Structure of a conserved domain common to the transcription factors TFIIS, Elongin A, and CRSP70. J Biol Chem 275:31266–31268

    Article  Google Scholar 

  • Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfizer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277293

    Article  Google Scholar 

  • Goddard TD, Kneller DG (1993) SPARKY3. University of California, San Francisco

    Google Scholar 

  • Kettenberger H, Armache KJ, Cramer P (2003) Architecture of the RNA polymerase II–TFIIS complex and implications for mRNA cleavage. Cell 114(3):347–357

    Article  Google Scholar 

  • Kim B, Nesvizhskii AI, Geetha-Rani P, Hahn S, Aebersold R, Ranish JA (2007) The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes. Proc Natl Acad Sci USA 104(41):16068–16073

    Article  ADS  Google Scholar 

  • Langer D, Zillig W (1993) Putative tfIIs gene of Sulfolobus acidocaldarius encoding an archaeal transcription elongation factor is situated directly downstream of the gene for a small subunit of DNA-dependent RNA polymerase. Nucleic Acids Res 21(9):2251

    Article  Google Scholar 

  • Morin PE, Awrey DE, Edwards AM, Arrowsmith CH (1996) Elongation factor TFIIS contains three structural domains: solution structure of domain II. Proc Natl Acad Sci USA 93(20):10604–10608

    Article  ADS  Google Scholar 

  • Pan G, Aso T, Greenblatt J (1997) Interaction of elongation factors TFIIS and Elongin A with a human RNA polymerase II holoenzyme capable of promoter-specific initiation and responsive to transcriptional activators. J Biol Chem 272:24563–24571

    Article  Google Scholar 

  • Qian X, Jeon C, Yoon H, Agarwal K, Weiss MA (1993a) Structure of a new nucleic-acid-binding motif in eukaryotic transcriptional elongation factor TFIIS. Nature 365(6443):277–279

    Article  ADS  Google Scholar 

  • Qian X, Gozani SN, Yoon H, Jeon CJ, Agarwal K, Weiss MA (1993b) Novel zinc finger motif in the basal transcriptional machinery: three-dimensional NMR studies of the nucleic acid binding domain of transcriptional elongation factor TFIIS. Biochemistry 32(38):9944–9959

    Article  Google Scholar 

  • Wishart DS, Sykes BD (1994) The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data. J Biomol NMR 4:171–180

    Article  Google Scholar 

Download references

Acknowledgements

We thank F. Delaglio and A. Bax for providing NMRPipe and NMRDraw and also thank Goddard and Kneller for providing SPARKY software. This work was supported by Fundamental Research Funds for the Central Universities (Grant No. WK9110000042), the Major/Innovative Program of the Development Foundation of the Hefei Center for Physical Science and Technology (Grant No. 2018cxfx007).

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Correspondence to Xiaoming Tu.

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Guo, H., Zhang, J., Gao, J. et al. 1H, 13C and 15N resonance assignments of TFIIS LW domain from Homo sapiens. Biomol NMR Assign 14, 201–203 (2020). https://doi.org/10.1007/s12104-020-09945-8

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  • DOI: https://doi.org/10.1007/s12104-020-09945-8

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