Human linker histone H1 plays a seminal role in eukaryotic DNA packaging. H1 has a tripartite structure consisting of a central, conserved globular domain, which adopts a winged-helix fold, flanked by two variable N- and C-terminal domains. Here we present the backbone resonance assignments of the N-terminal domain and globular domain of human linker histone H1x in the presence and absence of the secondary structure stabilizer sodium perchlorate. Analysis of chemical shift changes between the two conditions is consistent with induction of transient secondary structural elements in the N-terminal domain of H1x in high ionic strength, which suggests that the N-terminal domain adopts significant alpha-helical conformations in the presence of DNA.
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Financial support from the IR-RMN-THC FR 3050 CNRS (France) and NRF TTK14052667899 (South Africa) for conducting this research is gratefully acknowledged.
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de Wit, H., Vallet, A., Brutscher, B. et al. NMR assignments of human linker histone H1x N-terminal domain and globular domain in the presence and absence of perchlorate. Biomol NMR Assign 13, 249–254 (2019). https://doi.org/10.1007/s12104-019-09886-x
- Human linker histone H1x
- Intrinsically disordered protein
- Chromatin organization
- NMR backbone resonance assignment