Chemical shift assignments of retinal degeneration 3 protein (RD3)
Retinal degeneration 3 protein (RD3) binds to retinal membrane guanylyl cyclase (RetGC) and suppresses the basal activity of RetGC in photoreceptor cells that opposes the allosteric activation of the cyclase by GCAP proteins. Mutations in RD3 that disrupt its inhibition of RetGC are implicated in human retinal degenerative disorders. Here we report both backbone and sidechain NMR assignments for the RD3 protein (BMRB accession no. 27305).
KeywordsRetinal degeneration protein 3 Retinal guanylyl cyclase RetGC RD3 GCAP
We thank Jeff Walton for technical support and help with NMR experiments. Work supported by NIH Grants (EY012347) to J.B.A and (EY011522) to A.M.D.
- Friedman JS, Chang B, Kannabiran C, Chakarova C, Singh HP, Jalali S, Hawes NL, Branham K, Othman M, Filippova E, Thompson DA, Webster AR, Andréasson S, Jacobson SG, Bhattacharya SS, Heckenlively JR, Swaroop A (2006) Premature truncation of a novel protein, RD3, exhibiting subnuclear localization is associated with retinal degeneration. Am J Hum Genet 79:1059–1070CrossRefGoogle Scholar