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1H, 13C, and 15N resonance assignments of the cytokine interleukin-36β isoform-2

Biomolecular NMR Assignments volume 13, pages 155–161 (2019)Cite this article

Abstract

Interleukins are cytokines performing central tasks in the human immune system. Interleukin-36β (IL-36β) is a member of the interleukin-1 superfamily as are its homologues IL-36α and IL-36γ. All of them interact with a common receptor composed of IL-36R and IL-1R/acP. IL-36 cytokines can activate IL-36R to proliferation of CD4 + lymphocytes or stimulate M2 macrophages as potently as IL-1β. Within our efforts to study the structure–function relationship of the three interleukins IL-36α, IL-36β and IL-36γ by heteronuclear multidimensional NMR, we here report the 1H, 13C, and 15N resonance assignments for the backbone and side chain nuclei of cytokine interleukin-36β isoform-2.

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Notes

  1. CCPNmr derived numbers for the γ/δ/ε-positions include aromatic residues for which currently no assignment was attempted.

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Acknowledgements

Financial support by the Deutsche Forschungsgemeinschaft (DFG) within FOR 1738 (to D.I. and O.O.) is gratefully acknowledged. The FLI is a member of the Leibniz Association (WGL) and is financially supported by the Federal Government of Germany and the State of Thuringia. We thank Prof. Seamus Martin, Smurfit Institute of Genetics, Trinity College Dublin, Ireland for providing the IL-36β plasmid.

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Authors and Affiliations

  1. Leibniz Institute on Aging – Fritz Lipmann Institute, Beutenbergstr. 11, 07745, Jena, Germany

    Amit Kumar, Andras Lang, Ramadurai Ramachandran & Oliver Ohlenschläger

  2. Faculty of Chemistry and Earth Sciences, Friedrich Schiller University, Humboldtstr. 10, 07743, Jena, Germany

    Peter Bellstedt

  3. Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 3a, 06120, Halle/Saale, Germany

    Christoph Wiedemann

  4. Pharmaceutical Biochemistry and Bioanalytics, Pharmaceutical Institute, University of Bonn, An der Immenburg 4, 53121, Bonn, Germany

    Amelie Wißbrock & Diana Imhof

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  1. Amit Kumar
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  2. Amelie Wißbrock
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Correspondence to Oliver Ohlenschläger.

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In memory of Frank Große.

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Kumar, A., Wißbrock, A., Bellstedt, P. et al. 1H, 13C, and 15N resonance assignments of the cytokine interleukin-36β isoform-2. Biomol NMR Assign 13, 155–161 (2019). https://doi.org/10.1007/s12104-018-09869-4

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  • DOI: https://doi.org/10.1007/s12104-018-09869-4

Keywords

  • Resonance assignments
  • Heteronuclear NMR
  • Interleukin-36β
  • Interleukin-1 superfamily
  • Cytokines