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Biomolecular NMR Assignments

, Volume 12, Issue 1, pp 133–137 | Cite as

Backbone resonance assignment of the response regulator protein PhoBNF20D from Escherichia coli

  • Xinhui Kou
  • Xinghong Liu
  • Yixiang Liu
  • Conggang Li
  • Maili Liu
  • Ling Jiang
Article
  • 195 Downloads

Abstract

PhoB is a response regulator of the PhoR/PhoB two-component signal transduction system that is involved in the regulation of the phosphate (Pho) regulon of Escherichia coli. PhoB has two domains, receiver domain and effector domain. The receiver domain can be phosphorylated by its cognate histidine kinase PhoR and the phosphorylation induces conformational changes of the full length protein of PhoB that promote the DNA binding and transcription. Three-dimensional crystal structures of PhoB receiver domain (PhoBN) have been solved under apo or BeF3 (a phosphoryl analog) binding forms and it has been found that PhoBN is dimerized in both situations. However, we have found that the apo form of PhoBN has multiple conformational changes in solution that is hard to be distinguished by using NMR spectroscopy, while the mutagenesis of F20D PhoBN gives homogeneous dispersed signals in HSQC spectrum indicating a relatively uniform conformation. Meanwhile the F20D mutant has the same phosphorylation activity as the wild type protein. Here we report the backbone assignment of PhoBNF20D mutant. The chemical shift (HN, N, CO, Cα and Cβ) analysis shows that the predicted regions of secondary structure are in good agreement with those observed in the crystal structure of apo PhoBN. Therefore, the backbone chemical shifts assignment of PhoBNF20D mutant would be useful for studying the structure and dynamics of PhoB receiver domain and it has significance for explaining the mechanism of phosphorylation in TCSs.

Keywords

Chemical shift assignment NMR PhoBNF20D Secondary structure HSQC 

Notes

Acknowledgements

This project was supported by grants from the Natural Science Foundation of China # 21573280 and # 21603268.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Magnetic Resonance in Biological Systems, National Center for Magnetic Resonance in Wuhan, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and MathematicsThe Chinese Academy of SciencesWuhanChina
  2. 2.Graduate University of Chinese Academy of ScienceBeijingChina

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