Single stranded guanine rich DNA (or RNA) sequences adopt noncanonical secondary structures called G-quadruplexes (G4). Functionally, quadruplexes control gene transcription and regulate activities such as replication, gene recombination or alternative splicing. Hence they are potential targets for cancer, neuronal, and viral related diseases. KRAS is one of the most mutated oncogenes in the genome of cancer cells and contains a nuclease hypersensitive element (NHE) sequence capable of forming G-quadruplexes via its six runs of guanines. In our work, we are interested in the NMR structure of the major G4 scaffold formed in the KRAS NHE region with a mutated sequence of 22 residues. Here, we report 1H, 13C and 15N chemical shift assignments the G4 formed within KRAS22RT sequence.
KRAS oncogene Promoter region G-quadruplex Structure resonance assignments
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This work was possible thanks to financial supports from the IR-RMN THC Fr3050 CNRS facilities from Bordeaux (UMS3033/US001) and Gif-sur-Yvette. This work was also supported by iNEXT, Project Number 653706, funded by the Horizon 2020 from the European Union (Brno; Czech Republic). We are particularly grateful to R. Fiala from CEITEC for his help and his advices. Funds for conducting the research were also available from La Ligue contre le Cancer which we gratefully acknowledge. We also thank B. Vialet for synthesis of isotopically labeled oligonucleotides and our team for useful discussions.
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