Biomolecular NMR Assignments

, Volume 12, Issue 1, pp 51–55 | Cite as

Sequence specific 1H, 13C and 15N resonance assignments of a cataract-related variant G57W of human γS-crystallin

  • Khandekar Jishan Bari
  • Shrikant Sharma
  • Kandala V. R. Chary
Article
  • 88 Downloads

Abstract

γS-crystallin is a major structural component of the human eye lens, which maintains its stability over the lifetime of an organism with negligible turnover. The G57W mutant of human γS-crystallin (abbreviated hereafter as γS-G57W) is associated with dominant congenital cataracts. In order to provide a structural basis for the ability of γS-G57W causing cataract, we have cloned, overexpressed, isolated and purified the protein. The 2D [15N–1H]-HSQC spectrum recorded with uniformly 13C/15N-labelled γS-G57W was highly dispersed indicating the protein to adopt an ordered conformation. In this paper, we report almost complete sequence-specific 1H, 13C and 15N resonance assignments of γS-G57W using a suite of heteronuclear 3D NMR experiments.

Keywords

Crystallin Eye lens Cataract Greek key motifs Complete NMR assignments 

Abbreviations

γS-G57W

G57W mutant of human γS-crystallin

HSQC

Heteronuclear single quantum correlation

NMR

Nuclear magnetic resonance

Notes

Acknowledgements

The facilities provided by the National Facility for High Field NMR, supported by the Department of Science and Technology (DST), Department of Biotechnology (DBT), Council of Scientific and Industrial Research (CSIR), Tata Institute of Fundamental Research (TIFR), Mumbai and the NMR Centre at Indian Institute of Chemical Technology (IICT), Hyderabad are gratefully acknowledged. KVRC acknowledges the financial support of JC Bose fellowship of DST (Govt. of India).

Author contributions

KJB and SS have contributed equally to this work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Center for Interdisciplinary SciencesTata Institute of Fundamental ResearchHyderabadIndia
  2. 2.Department of Chemical SciencesTata Institute of Fundamental ResearchMumbaiIndia

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