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Biomolecular NMR Assignments

, Volume 12, Issue 1, pp 23–26 | Cite as

1H, 13C and 15N assignments of the C-terminal intrinsically disordered cytosolic fragment of the receptor tyrosine kinase ErbB2

  • YingHui Wang
  • Louise Pinet
  • Nadine Assrir
  • Latifa Elantak
  • Françoise Guerlesquin
  • Ali Badache
  • Ewen Lescop
  • Carine van Heijenoort
Article

Abstract

ErbB2 (or HER2) is a receptor tyrosine kinase that is involved in signaling pathways controlling cell division, motility and apoptosis. Though important in development and cell growth homeostasis, this protein, when overexpressed, participates in triggering aggressive HER2+ breast cancers. It is composed of an extracellular part and a transmembrane domain, both important for activation by dimerization, and a cytosolic tyrosine kinase, which activates its intrinsically disordered C-terminal end (CtErbB2). Little is known about this C-terminal part of 268 residues, despite its crucial role in interacting with adaptor proteins involved in signaling. Understanding its structural and dynamic characteristics could eventually lead to the design of new interaction inhibitors, and treatments complementary to those already targeting other parts of ErbB2. Here we report backbone and side-chain assignment of CtErbB2, which, together with structural predictions, confirms its intrinsically disordered nature.

Keywords

Receptor tyrosine kinase ErbB2 Intrinsically disordered protein Breast cancer 

Notes

Acknowledgements

We thank the French National Research Agency (ANR) (research Grant ANR-13-BSV8-0016 to CvH and financing YW) for providing financial support to the project. Louise Pinet is funded by a Ph.D Grant from the Université Paris-Sud. Financial support from the TGIR-RMN-THC Fr3050 CNRS for conducting the research is gratefully acknowledged.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Institut de Chimie des Substances Naturelles, Structural Chemistry and Biology teamCNRS, Université Paris-SaclayGif-sur-YvetteFrance
  2. 2.Laboratoire d’Ingénierie des Systèmes MacromoléculairesCNRS UMR 7255MarseilleFrance
  3. 3.Centre de Recherche en Cancérologie de Marseille, INSERM U 1068MarseilleFrance

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