Biomolecular NMR Assignments

, Volume 12, Issue 1, pp 5–9 | Cite as

1H, 15N and 13C sequence specific backbone assignment of the vanadate inhibited hematopoietic tyrosine phosphatase

  • Luciana E. S. F. Machado
  • Rebecca Page
  • Wolfgang Peti
Article
  • 103 Downloads

Abstract

The sequence-specific backbone assignment of hematopoietic protein tyrosine phosphatase (HePTP; PTPN7) in presence of vanadate has been determined, based on triple-resonance experiments using uniformly [13C,15N]-labeled protein. These assignments facilitate further studies of HePTP in the presence of inhibitors to target leukemia and provide further insights into the function of protein tyrosine phosphatases.

Keywords

HePTP Protein tyrosine phosphatase Vanadate Inhibition Protein dynamics Leukemia 

Notes

Acknowledgements

This work was supported by the American Diabetes Association Pathway to Stop Diabetes Grant 1-14-ACN-31 and a Brazil Initiative Brown University grant to WP and NIH R01GM098482 to RP.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Luciana E. S. F. Machado
    • 1
    • 3
  • Rebecca Page
    • 2
    • 3
  • Wolfgang Peti
    • 1
    • 3
  1. 1.Department of Molecular Pharmacology, Physiology and BiotechnologyBrown UniversityProvidenceUSA
  2. 2.Department of Molecular Biology, Cell Biology and BiochemistryBrown UniversityProvidenceUSA
  3. 3.Department of Chemistry and BiochemistryUniversity of ArizonaTucsonUSA

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