Abstract
Conjugative transfer of DNA represents the most important transmission pathway in terms of antibiotic resistance and virulence gene dissemination among bacteria. TraH is a putative transfer protein of the type IV secretion system (T4SS) encoded by the Gram-positive (G+) conjugative plasmid pIP501. This molecular machine involves a multi-protein core complex spanning the bacterial envelope thereby serving as a macromolecular secretion channel. Here, we report the near complete 1H, 13C and 15N resonance assignment of a soluble TraH variant comprising the C-terminal domain.
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Acknowledgments
Funding by the Austrian Science Foundation (FWF) under Project Number P27383 to W.K. is gratefully acknowledged. Further funding in support of C.F. was provided by the University of Graz as part of the Incentive Initiative for the resubmission of excellent FWF projects.
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Fercher, C., Keller, W., Zangger, K. et al. 1H, 15N and 13C chemical shift assignment of the Gram-positive conjugative transfer protein TraHpIP501 . Biomol NMR Assign 10, 163–166 (2016). https://doi.org/10.1007/s12104-015-9658-3
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DOI: https://doi.org/10.1007/s12104-015-9658-3