Biomolecular NMR Assignments

, Volume 9, Issue 2, pp 261–265 | Cite as

NMR assignments of actin depolymerizing factor (ADF) like UNC-60A and cofilin like UNC-60B proteins of Caenorhabditis elegans

  • Vaibhav Kumar Shukla
  • Ashish Kabra
  • Rahul Yadav
  • Shoichiro Ono
  • Dinesh Kumar
  • Ashish AroraEmail author


The actin filament dynamics in nematode, Caenorhabditis elegans, is regulated by differential activity of two proteins UNC-60A and UNC-60B. UNC-60A exhibits strong pointed end depolymerization on C. elegans actin (Ce-actin), strong inhibition of polymerization, strong monomer sequestering activity, weak severing activity, and low affinity for F-actin binding, while UNC-60B exhibits strong pointed end depolymerization on rabbit muscle actin, strong severing activity, and high affinity for F-actin binding. Structural characterization of these proteins will help to understand (1) molecular mechanism of actin dynamics regulation and (2) the differential activity of these proteins. Here, we report 1H, 13C, and 15N chemical shift assignments of these two proteins as determined by heteronuclear NMR experiments (at pH 6.5 and temperature 298 K).


NMR Resonance assignment ADF like protein UNC-60A UNC-60B 



We are thankful to Dr. C.L. Khetrapal for usage of 800 MHz NMR spectrometer at the Centre for Biomedical Research, Lucknow. This work was supported by Council of Scientific and Industrial Research (CSIR) Network Project UNSEEN, and National Biosciences Award Grant from Department of Biotechnology (DBT), to AA. V.K.S. is a recipient of research fellowship from CSIR, New Delhi, India. R.Y. and A.K. are recipients of research fellowship from DBT and Indian Council for Medical Research, New Delhi, India, respectively. Work relating to unc-60a and unc-60b clones was supported by a Grant from National Institutes of Health (AR048615) to S.O.


  1. Alfano C, Babon J, Kelly G, Curry S, Conte MR (2003) Resonance assignment and secondary structure of an N-terminal fragment of the human La protein. J Biomol NMR 27:93–94CrossRefGoogle Scholar
  2. Cornilescu G, Delaglio F, Bax A (1999) Protein backbone angle restraints from searching a database for chemical shift and sequence homology. J Biomol NMR 13:289–302CrossRefGoogle Scholar
  3. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277–293CrossRefGoogle Scholar
  4. Keller R (2004) The computer aided resonance assignment tutorial CANTINA. Verlag, GoldauGoogle Scholar
  5. Ono S (2007) Mechanism of depolymerization and severing of actin filaments and its significance in cytoskeletal dynamics. Int Rev Cytol 258:1–82CrossRefGoogle Scholar
  6. Ono S, Benian GM (1998) Two Caenorhabditis elegans actin depolymerizing factor/cofilin proteins, encoded by the unc-60 gene, differentially regulate actin filament dynamics. J Biol Chem 273:3778–3783CrossRefGoogle Scholar
  7. Ono S, Baillie DL, Benian GM (1999) UNC-60B, an ADF/cofilin family protein, is required for proper assembly of actin into myofibrils in Caenorhabditis elegans body wall muscle. J Cell Biol 145:491–502CrossRefGoogle Scholar
  8. Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44:213–223CrossRefGoogle Scholar
  9. Yamashiro S, Mohri K, Ono S (2005) The two Caenorhabditis elegans actin-depolymerizing factor/cofilin proteins differently enhance actin filament severing and depolymerization. Biochemistry 44:14238–14247CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Vaibhav Kumar Shukla
    • 1
  • Ashish Kabra
    • 1
  • Rahul Yadav
    • 1
  • Shoichiro Ono
    • 3
    • 4
  • Dinesh Kumar
    • 2
  • Ashish Arora
    • 1
    Email author
  1. 1.Molecular and Structural Biology DivisionCSIR-Central Drug Research InstituteLucknowIndia
  2. 2.Centre of Biomedical ResearchLucknowIndia
  3. 3.Department of PathologyEmory UniversityAtlantaUSA
  4. 4.Department of Cell BiologyEmory UniversityAtlantaUSA

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