Backbone 1H, 15N, 13C and side chain 13Cβ NMR chemical shift assignment of murine interleukin-10
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Almost complete assignment of backbone 1H, 13C, 15N and side chain 13Cβ resonances for the immune-regulatory cytokine IL-10 is reported. The protein was overexpressed in Escherichia coli and was refolded from inclusion bodies. The point mutation C149Y was introduced to suppress incorrect disulfide bond formation and to improve protein refolding.
KeywordsCytokine Autoimmunity NMR Protein-carbohydrate-interaction
We thank Sebastian Lanvermann (Technical University Dresden) who provided the IL-10 cDNA and Professors Stefan Berger (University of Leipzig) and Jochen Balbach (Martin Luther University Halle/Wittenberg) who kindly provided access to their NMR spectrometers. The study was supported by a grant from the Deutsche Forschungsgemeinschaft (Transregio 67, A6) and a grant from the Stipendienfonds der Chemischen Industrie e.V.
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