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Biomolecular NMR Assignments

, Volume 8, Issue 2, pp 345–348 | Cite as

Backbone and side chain NMR assignments for the ribosome assembly factor Nop6 from Saccharomyces cerevisiae

  • Jan Philip Wurm
  • Anatoli Lioutikov
  • Peter Kötter
  • Karl-Dieter Entian
  • Jens WöhnertEmail author
Article
  • 173 Downloads

Abstract

The Saccharomyces cerevisiae Nop6 protein is involved in the maturation of the small ribosomal subunit. It contains a central RNA binding domain and a predicted C-terminal coiled-coil domain. Here we report the almost complete (>90 %) 1H,13C,15N backbone and side chain NMR assignment of a 15 kDa Nop6 construct comprising the RNA binding and coiled-coil domains.

Keywords

NMR-assignments Triple resonance experiments RNA binding domain Ribosome assembly 

Notes

Acknowledgments

We are grateful to Dr. Elke Duchardt-Ferner for helpful discussions. This project was supported by an Aventis Foundation Professorship (to J.W.), the Center of Biomolecular Magnetic Resonance, Johann-Wolfgang-Goethe-University Frankfurt, and the Deutsche Forschungsgemeinschaft (DFG) through the SFB 902 ‘Molecular Principles of RNA-based Regulation’.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Jan Philip Wurm
    • 1
    • 2
  • Anatoli Lioutikov
    • 1
  • Peter Kötter
    • 1
  • Karl-Dieter Entian
    • 1
  • Jens Wöhnert
    • 1
    • 2
    Email author
  1. 1.Institut für Molekulare BiowissenschaftenJohann-Wolfgang-Goethe-Universität Frankfurt/MFrankfurtGermany
  2. 2.Center for Biomolecular Magnetic Resonance (BMRZ)Johann-Wolfgang-Goethe-UniversitätFrankfurtGermany

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