Backbone and side chain NMR assignments for the ribosome assembly factor Nop6 from Saccharomyces cerevisiae
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The Saccharomyces cerevisiae Nop6 protein is involved in the maturation of the small ribosomal subunit. It contains a central RNA binding domain and a predicted C-terminal coiled-coil domain. Here we report the almost complete (>90 %) 1H,13C,15N backbone and side chain NMR assignment of a 15 kDa Nop6 construct comprising the RNA binding and coiled-coil domains.
KeywordsNMR-assignments Triple resonance experiments RNA binding domain Ribosome assembly
We are grateful to Dr. Elke Duchardt-Ferner for helpful discussions. This project was supported by an Aventis Foundation Professorship (to J.W.), the Center of Biomolecular Magnetic Resonance, Johann-Wolfgang-Goethe-University Frankfurt, and the Deutsche Forschungsgemeinschaft (DFG) through the SFB 902 ‘Molecular Principles of RNA-based Regulation’.
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