1H, 15N, and 13C chemical shift assignments of cyanobacteriochrome NpF2164g3 in the photoproduct state


Cyanobacteriochrome (CBCR) photosensory proteins are phytochrome relatives using bilin chromophores for light sensing across the visible spectrum. Structural information is not available for two of the four known CBCR subfamilies. NpF2164g3 is a member of one such subfamily, exhibiting a violet/orange photocycle. We report backbone NMR chemical shift assignments for the light-activated orange-absorbing state of NpF2164g3 (BMRB no. 19150).

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We thank Jerry Dallas for technical support and help with NMR experiments. This work was supported by a grant from the Chemical Sciences, Geosciences, and Biosciences Division, Office of Basic Energy Sciences, Office of Science, United States Department of Energy (DOE DE-FG02-09ER16117 to J.C.L. and J.B.A.), with partial support for NMR time from NIH grant RR11973 to the UC Davis NMR facility. Pilot experiments were supported by NIH grant EY012347 to J.B.A.

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Correspondence to James B. Ames.

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Lim, S., Rockwell, N.C., Martin, S.S. et al. 1H, 15N, and 13C chemical shift assignments of cyanobacteriochrome NpF2164g3 in the photoproduct state. Biomol NMR Assign 8, 259–262 (2014). https://doi.org/10.1007/s12104-013-9496-0

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  • Photoreceptor
  • CBCR
  • Phytochrome
  • Cyanobacteria
  • Tetrapyrrole
  • NMR