Abstract
Cyanobacteriochrome (CBCR) photosensory proteins are phytochrome relatives using bilin chromophores for light sensing across the visible spectrum. Structural information is not available for two of the four known CBCR subfamilies. NpF2164g3 is a member of one such subfamily, exhibiting a violet/orange photocycle. We report backbone NMR chemical shift assignments for the light-activated orange-absorbing state of NpF2164g3 (BMRB no. 19150).
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Auldridge ME, Forest KT (2011) Bacterial phytochromes: more than meets the light. Crit Rev Biochem Mol Biol 46(1):67–88
Delaglio F, Grzesiek S et al (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6(3):277–293
Ikeuchi M, Ishizuka T (2008) Cyanobacteriochromes: a new superfamily of tetrapyrrole-binding photoreceptors in cyanobacteria. Photochem Photobiol Sci 7(10):1159–1167
Ikura M, Kay LE et al (1990) A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin. Biochemistry 29(19):4659–4667
Ishizuka T, Kamiya A et al (2011) The cyanobacteriochrome, TePixJ, isomerizes its own chromophore by converting phycocyanobilin to phycoviolobilin. Biochemistry 50(6):953–961
Narikawa R, Ishizuka T et al (2013) Structures of cyanobacteriochromes from phototaxis regulators AnPixJ and TePixJ reveal general and specific photoconversion mechanism. Proc Natl Acad Sci 110(3):918–923
Rockwell NC, Martin SS et al (2011) Diverse two-cysteine photocycles in phytochromes and cyanobacteriochromes. Proc Natl Acad Sci 108(29):11854–11859
Rockwell NC, Martin SS et al (2012) Phycoviolobilin formation and spectral tuning in the DXCF cyanobacteriochrome subfamily. Biochemistry 51:1449–1463
Wishart DS, Sykes BD et al (1992) The chemical shift index: a fast and simple method for the assignment of protein secondary structure through NMR spectroscopy. Biochemistry 31(6):1647–1651
Acknowledgments
We thank Jerry Dallas for technical support and help with NMR experiments. This work was supported by a grant from the Chemical Sciences, Geosciences, and Biosciences Division, Office of Basic Energy Sciences, Office of Science, United States Department of Energy (DOE DE-FG02-09ER16117 to J.C.L. and J.B.A.), with partial support for NMR time from NIH grant RR11973 to the UC Davis NMR facility. Pilot experiments were supported by NIH grant EY012347 to J.B.A.
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Lim, S., Rockwell, N.C., Martin, S.S. et al. 1H, 15N, and 13C chemical shift assignments of cyanobacteriochrome NpF2164g3 in the photoproduct state. Biomol NMR Assign 8, 259–262 (2014). https://doi.org/10.1007/s12104-013-9496-0
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DOI: https://doi.org/10.1007/s12104-013-9496-0