Streptococcus pneumoniae adhesin competence repressor (AdcR) is a Zn(II)-dependent 32 kDa homodimer that controls the transcription of a zinc-specific ABC uptake system (AdcABC), three pneumococcal histidine triad proteins (PhtA, PhtD and PhtE), and an AdcA homolog AdcAII. AdcR is the first metal-dependent member of the MarR family of prokaryotic transcriptional repressors. Two-dimensional NMR studies reveal large changes in the spectrum upon Zn(II) binding. Near complete backbone and stereospecific methyl group resonance assignments for apo- and Zn(II)-AdcR are presented here.
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This work was supported by the NIH grant GM042569 (to D.P.G.).
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The authors declare that they have no conflict of interest.
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Guerra, A.J., Giedroc, D.P. Backbone and sterospecific methyl side chain resonance assignments of the homodimeric zinc sensor AdcR (32 kDa) in the apo- and Zn(II)-bound states. Biomol NMR Assign 8, 11–14 (2014). https://doi.org/10.1007/s12104-012-9442-6
- Metalloregulatory protein
- Winged helix
- Zinc sensor
- Allosteric regulation
- S. pneumoniae